Literature DB >> 14715316

PCR-generated artificial ribosomal DNAs from premature termination at Alu sequences.

N S Kupriyanova1, D V Shibalev, A S Voronov, A P Ryskov.   

Abstract

PCR-amplified product may sometimes not correlate with a DNA state in vivo due to formation of recombinant molecules. Here we show that recombinant product can form in vitro on amplifying the region upstream of the rRNA transcription start point in human ribosomal intergenic spacer. These results provide the first information concerning definite Alu sites where premature polymerase termination occurs.

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Year:  2004        PMID: 14715316     DOI: 10.1016/s1389-0344(03)00004-2

Source DB:  PubMed          Journal:  Biomol Eng        ISSN: 1389-0344


  1 in total

1.  Characteristics of the structure of minor variants of external transcribed spacers of ribosomal DNA of the cockroach Blattella germanica.

Authors:  D V Mukha; V A Mysina; C Schal
Journal:  Dokl Biol Sci       Date:  2005 Sep-Oct
  1 in total

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