| Literature DB >> 14715313 |
Florence Komurian-Pradel1, Magali Perret, Birgit Deiman, Mireille Sodoyer, Vincent Lotteau, Glaucia Paranhos-Baccalà, Patrice André.
Abstract
Qualitative detection of negative hepatitis C virus (HCV) RNA has been used widely to demonstrate HCV replication. However, relative quantitation of both positive and negative HCV RNA strands has never been reported for studying viral genome replication. A strand specific real-time PCR carried out in the highly conserved 5'-non-coding region of HCV genome and monitored either by the DNA binding dye SYBR Green I or by molecular beacons is described. Using these techniques, it was found that negative HCV RNA strand was a 100-1000 times less abundant than the positive strand in the liver of HCV infected patients.Entities:
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Year: 2004 PMID： 14715313 DOI： 10.1016/j.jviromet.2003.10.004
Source DB: PubMed Journal: J Virol Methods ISSN： 0166-0934 Impact factor: 2.014