| Literature DB >> 14715286 |
Antoine Viens1, Undine Mechold, Heike Lehrmann, Annick Harel-Bellan, Vasily Ogryzko.
Abstract
We describe a system designed to express biotinylated proteins in mammalian cells in vivo and its application to the study of protein-DNA interactions in vivo by chromatin immunoprecipitation (ChIP). The system is based on coexpression of the target protein fused to a short biotin acceptor domain together with the biotinylating enzyme BirA from Escherichia coli. The superior strength of the biotin-avidin interaction allows one to employ more stringent washing conditions in the ChIP protocol, resulting in a better signal/noise ratio.Entities:
Mesh：
Substances：
Year: 2004 PMID： 14715286 DOI： 10.1016/j.ab.2003.10.015
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365