| Literature DB >> 14713171 |
Teruhiko Nitoda1, Hirokazu Usuki, Hiroshi Kanzaki.
Abstract
A water-soluble polysaccharide was isolated from the culture filtrate of a fungal strain, Sphaeropsis sp. TNPT116-Cz, as a novel insect chitinase inhibitor. It was purified to chromatographic homogeneity by ethanol precipitation, anion-exchange and gel filtration chromatography. Its molecular weight was estimated to be 16 kDa by gel filtration HPLC. Monosaccharide analysis showed that it contained glucose, galactose, N-acetylglucosamine and a deoxysugar. This polysaccharide showed potent and specific inhibitory activity against Spodoptera litura chitinase with an IC50 value of 28 nM.Entities:
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Year: 2003 PMID： 14713171 DOI： 10.1515/znc-2003-11-1226
Source DB: PubMed Journal: Z Naturforsch C J Biosci ISSN： 0341-0382