Literature DB >> 14711700

Molecular cloning, purification, and biochemical characterization of hydantoin racemase from the legume symbiont Sinorhizobium meliloti CECT 4114.

Sergio Martínez-Rodríguez1, Francisco Javier Las Heras-Vázquez, Lydia Mingorance-Cazorla, Josefa María Clemente-Jiménez, Felipe Rodríguez-Vico.   

Abstract

Hydantoin racemase from Sinorhizobium meliloti was functionally expressed in Escherichia coli. The native form of the enzyme was a homotetramer with a molecular mass of 100 kDa. The optimum temperature and pH for the enzyme were 40 degrees C and 8.5, respectively. The enzyme showed a slight preference for hydantoins with short rather than long aliphatic side chains or those with aromatic rings. Substrates, which showed no detectable activity toward the enzyme, were found to exhibit competitive inhibition.

Entities:  

Mesh：

Substances：

Year:  2004        PMID： 14711700      PMCID： PMC321266          DOI： 10.1128/AEM.70.1.625-630.2004

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  12 in total

1.  Complete conversion of D,L-5-monosubstituted hydantoins with a low velocity of chemical racemization into D-amino acids using whole cells of recombinant Escherichia coli.

Authors:  Sergio Martinez-Rodriguez; Francisco Javier Las Heras-Vazquez; Josefa María Clemente-Jimenez; Lydia Mingorance-Cazorla; Felipe Rodriguez-Vico
Journal:  Biotechnol Prog       Date:  2002 Nov-Dec

2.  The chemistry of the hydantoins.

Authors:  E WARE
Journal:  Chem Rev       Date:  1950-06       Impact factor: 60.622

3.  Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021.

Authors:  D Capela; F Barloy-Hubler; J Gouzy; G Bothe; F Ampe; J Batut; P Boistard; A Becker; M Boutry; E Cadieu; S Dréano; S Gloux; T Godrie; A Goffeau; D Kahn; E Kiss; V Lelaure; D Masuy; T Pohl; D Portetelle; A Pühler; B Purnelle; U Ramsperger; C Renard; P Thébault; M Vandenbol; S Weidner; F Galibert
Journal:  Proc Natl Acad Sci U S A       Date:  2001-07-31       Impact factor: 11.205

4.  Organization of genes responsible for the stereospecific conversion of hydantoins to alpha-amino acids in Arthrobacter aurescens DSM 3747.

Authors:  A Wiese; C Syldatk; R Mattes; J Altenbuchner
Journal:  Arch Microbiol       Date:  2001-09       Impact factor: 2.552

5.  Hydantoin racemase from Arthrobacter aurescens DSM 3747: heterologous expression, purification and characterization.

Authors:  A Wiese; M Pietzsch; C Syldatk; R Mattes; J Altenbuchner
Journal:  J Biotechnol       Date:  2000-07-14       Impact factor: 3.307

6.  Cloning and characterization of genes from Agrobacterium sp. IP I-671 involved in hydantoin degradation.

Authors:  M Hils; P Münch; J Altenbuchner; C Syldatk; R Mattes
Journal:  Appl Microbiol Biotechnol       Date:  2001-12       Impact factor: 4.813

7.  The genomes of the family Rhizobiaceae: size, stability, and rarely cutting restriction endonucleases.

Authors:  B W Sobral; R J Honeycutt; A G Atherly
Journal:  J Bacteriol       Date:  1991-01       Impact factor: 3.490

8.  Identification and sequencing of a gene encoding a hydantoin racemase from the native plasmid of Pseudomonas sp. strain NS671.

Authors:  K Watabe; T Ishikawa; Y Mukohara; H Nakamura
Journal:  J Bacteriol       Date:  1992-06       Impact factor: 3.490

9.  Purification and characterization of the hydantoin racemase of Pseudomonas sp. strain NS671 expressed in Escherichia coli.

Authors:  K Watabe; T Ishikawa; Y Mukohara; H Nakamura
Journal:  J Bacteriol       Date:  1992-12       Impact factor: 3.490

10.  Overexpression and characterization of hydantoin racemase from Agrobacterium tumefaciens C58.

Authors:  Francisco Javier Las Heras-Vázquez; Sergio Martínez-Rodríguez; Lydia Mingorance-Cazorla; Josefa María Clemente-Jiménez; Felipe Rodríguez-Vico
Journal:  Biochem Biophys Res Commun       Date:  2003-04-04       Impact factor: 3.575
View more
  6 in total

1.  The hydantoin transport protein from Microbacterium liquefaciens.

Authors:  Shun'ichi Suzuki; Peter J F Henderson
Journal:  J Bacteriol       Date:  2006-05       Impact factor: 3.490

2.  Recombinant polycistronic structure of hydantoinase process genes in Escherichia coli for the production of optically pure D-amino acids.

Authors:  Ana Isabel Martínez-Gómez; Sergio Martínez-Rodríguez; Josefa María Clemente-Jiménez; Joaquín Pozo-Dengra; Felipe Rodríguez-Vico; Francisco Javier Las Heras-Vázquez
Journal:  Appl Environ Microbiol       Date:  2007-01-12       Impact factor: 4.792

3.  Crystallization and preliminary crystallographic studies of an active-site mutant hydantoin racemase from Sinorhizobium meliloti CECT4114.

Authors:  Sergio Martínez-Rodríguez; Luis Antonio González-Ramírez; Josefa María Clemente-Jiménez; Felipe Rodríguez-Vico; Francisco Javier Las Heras-Vázquez; Jose Antonio Gavira; Juan Ma García-Ruiz
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2007-12-20

4.  Site-directed mutagenesis indicates an important role of cysteines 76 and 181 in the catalysis of hydantoin racemase from Sinorhizobium meliloti.

Authors:  Sergio Martínez-Rodríguez; Montserrat Andújar-Sánchez; Jose L Neira; Josefa M Clemente-Jiménez; Vicente Jara-Pérez; Felipe Rodríguez-Vico; Francisco J Las Heras-Vázquez
Journal:  Protein Sci       Date:  2006-12       Impact factor: 6.725

Review 5.  Norcoclaurine synthase: mechanism of an enantioselective pictet-spengler catalyzing enzyme.

Authors:  Alessandra Bonamore; Marco Barba; Bruno Botta; Alberto Boffi; Alberto Macone
Journal:  Molecules       Date:  2010-03-24       Impact factor: 4.411

6.  Enzymatic enantioselective decarboxylative protonation of heteroaryl malonates.

Authors:  Ross Lewin; Mark Goodall; Mark L Thompson; James Leigh; Michael Breuer; Kai Baldenius; Jason Micklefield
Journal:  Chemistry       Date:  2015-03-12       Impact factor: 5.236
  6 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.