| Literature DB >> 14711596 |
Marleen H Roos1, Myrthe Otsen, Ruurdtje Hoekstra, Jetty G Veenstra, Johannes A Lenstra.
Abstract
Haemonchus contortus is a sheep parasitic nematode that causes severe economic losses. Previous studies have indicated a high degree of genetic heterogeneity, which is hardly affected by selection for drug resistance. As a tool for the analysis of the population dynamics of H. contortus and its response to drug resistance, we designed a strategy to study the inbreeding of a benzimidazole-sensitive and a benzimidazole-resistant strain. After 15 generations, a theoretical inbreeding coefficient of 0.87 was achieved. The different stages of inbreeding were analysed using restriction fragment polymorphism, microsatellite variability and amplified fragment length polymorphism. Model-based clustering of the amplified fragment length polymorphism genotypes showed that the allele frequencies of the benzimidazole-resistant strain were stable during the last eight generations. In the sensitive strain a gradual shift of allele frequencies was observed, which led to a temporary increase of the genetic diversity around the eight generations.Entities:
Mesh：
Substances：
Year: 2004 PMID： 14711596 DOI： 10.1016/j.ijpara.2003.10.002
Source DB: PubMed Journal: Int J Parasitol ISSN： 0020-7519 Impact factor: 3.981