BACKGROUND: Interleukin 6 (IL-6) is a pleiotropic cytokine that plays an essential role in the pathogenesis of acute and chronic infections. As the role of the IL-6 G(-174)C polymorphism in determining serum concentrations of IL-6 is controversial, we studied the genotype-specific IL-6 response in a well-standardized model of systemic inflammation. METHODS: A total of 76 healthy young males (age range, 19-35 years) received a single bolus of 2 ng/kg endotoxin [lipopolysaccharide (LPS)] intravenously. Plasma IL-6 was measured by enzyme immunoassay at 0, 2, 6, and 24 h after LPS infusion, and the IL-6 promoter genotype was analyzed by a mutagenic separated PCR assay. RESULTS: IL-6 increased 300-fold 2 h after LPS challenge and returned almost to normal within 24 h. Neither basal IL-6 nor the IL-6 response to LPS was significantly affected by the IL-6 promoter genotype. CONCLUSIONS: The IL-6 G(-174)C promoter polymorphism does not significantly influence basal concentrations of IL-6 or peak IL-6 in human endotoxemia.
BACKGROUND:Interleukin 6 (IL-6) is a pleiotropic cytokine that plays an essential role in the pathogenesis of acute and chronic infections. As the role of the IL-6 G(-174)C polymorphism in determining serum concentrations of IL-6 is controversial, we studied the genotype-specific IL-6 response in a well-standardized model of systemic inflammation. METHODS: A total of 76 healthy young males (age range, 19-35 years) received a single bolus of 2 ng/kg endotoxin [lipopolysaccharide (LPS)] intravenously. Plasma IL-6 was measured by enzyme immunoassay at 0, 2, 6, and 24 h after LPS infusion, and the IL-6 promoter genotype was analyzed by a mutagenic separated PCR assay. RESULTS:IL-6 increased 300-fold 2 h after LPS challenge and returned almost to normal within 24 h. Neither basal IL-6 nor the IL-6 response to LPS was significantly affected by the IL-6 promoter genotype. CONCLUSIONS: The IL-6 G(-174)C promoter polymorphism does not significantly influence basal concentrations of IL-6 or peak IL-6 in humanendotoxemia.
Authors: Stephanie L Rellick; Debbie Piktel; Cheryl Walton; Brett Hall; William Petros; James E Fortney; Marieta Gencheva; Jim Denvir; Gerald Hobbs; Michael Craig; Laura F Gibson Journal: Cytokine Date: 2012-02-21 Impact factor: 3.861
Authors: Florian M Kovar; Claudia L Marsik; Bernd Jilma; Christine Mannhalter; Christian Joukhadar; Oswald F Wagner; Georg Endler Journal: Wien Klin Wochenschr Date: 2009 Impact factor: 1.704
Authors: Seham F Azab; Mohamed A Abdalhady; Alshaymaa Ali; Ezzat K Amin; Dina T Sarhan; Eman M Elhindawy; Mohamed A A Almalky; Ahmed A Elhewala; Mohamed M A Salam; Mustafa I A Hashem; Attia A Soliman; Nagwa E Akeel; Sawsan H Abdellatif; Sanaa M Ismail; Nahla A Elsamad; Manal S Arafat; Anwar A Rass; Maha A A Basset Journal: Ital J Pediatr Date: 2016-03-09 Impact factor: 2.638
Authors: Srinivasan Beddhu; Rebecca Filipowicz; Bin Wang; Guo Wei; Xiaorui Chen; Abinash C Roy; Scott L DuVall; Hanadi Farrukh; Arsalan N Habib; Terrence Bjordahl; Debra L Simmons; Mark Munger; Greg Stoddard; Donald E Kohan; Tom Greene; Yufeng Huang Journal: Can J Kidney Health Dis Date: 2016-12-05