Literature DB >> 14706758

Photometric assay for measuring the intracellular concentration of branched-chain amino acids in bacteria.

Dina Petranovic1, Ivan Mijakovic.   

Abstract

The changes in intracellular pool of branched-chain amino acids (BCAA) regulate different physiological processes in bacteria. Up to date, the only available photometric test for measuring BCAA concentration was adapted for blood and plasma samples in diagnostic purposes. We have modified this method for use on bacterial cells, and tested its applicability on several model organisms: Lactococcus lactis, Bacillus subtilis and Escherichia coli.

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Year:  2004        PMID: 14706758     DOI: 10.1016/j.mimet.2003.10.002

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN: 0167-7012            Impact factor:   2.363


  3 in total

1.  Identification, source, and metabolism of N-ethylglutamate in Escherichia coli.

Authors:  Robert H White
Journal:  J Bacteriol       Date:  2010-08-13       Impact factor: 3.490

2.  Probing direct interactions between CodY and the oppD promoter of Lactococcus lactis.

Authors:  Chris D den Hengst; Peter Curley; Rasmus Larsen; Girbe Buist; Arjen Nauta; Douwe van Sinderen; Oscar P Kuipers; Jan Kok
Journal:  J Bacteriol       Date:  2005-01       Impact factor: 3.490

3.  A kinetic platform for in silico modeling of the metabolic dynamics in Escherichia coli.

Authors:  Aditya Barve; Anvita Gupta; Suresh M Solapure; Ansu Kumar; Vasanthi Ramachandran; Kothandaraman Seshadri; Shireen Vali; Santanu Datta
Journal:  Adv Appl Bioinform Chem       Date:  2010-12-07
  3 in total

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