PURPOSE: To determine the expression and distribution of tissue transglutaminase (TG(C)) and extracellular matrix (ECM) proteins in rat cornea during epithelial wound healing. METHODS: Corneal epithelial defects were created in rat corneas, and TG(C) expression was examined by Northern blot analysis, in situ hybridization, and immunohistochemical staining after the injury. The presence of fibrinogen, laminin-1, nidogen/entactin, and type collagen was also determined immunohistochemically. RESULTS: TG(C) was expressed in normal corneas. During the early wound healing process, TG(C) mRNA expression was up-regulated and TG(C) immunoreactivity was predominantly expressed in the migrating epithelial cells. ECM proteins were also expressed in a similar pattern as TG(C). CONCLUSIONS: The sites and time course of TG(C) expression indicate that TG(C) probably plays a role in maintaining the homeostasis of the cornea and in promoting epithelial wound healing. The simultaneous expression of TG(C) and ECM proteins suggests that the ECM proteins probably operate in concert with TG(C) in corneal wound healing.
PURPOSE: To determine the expression and distribution of tissue transglutaminase (TG(C)) and extracellular matrix (ECM) proteins in rat cornea during epithelial wound healing. METHODS:Corneal epithelial defects were created in rat corneas, and TG(C) expression was examined by Northern blot analysis, in situ hybridization, and immunohistochemical staining after the injury. The presence of fibrinogen, laminin-1, nidogen/entactin, and type collagen was also determined immunohistochemically. RESULTS: TG(C) was expressed in normal corneas. During the early wound healing process, TG(C) mRNA expression was up-regulated and TG(C) immunoreactivity was predominantly expressed in the migrating epithelial cells. ECM proteins were also expressed in a similar pattern as TG(C). CONCLUSIONS: The sites and time course of TG(C) expression indicate that TG(C) probably plays a role in maintaining the homeostasis of the cornea and in promoting epithelial wound healing. The simultaneous expression of TG(C) and ECM proteins suggests that the ECM proteins probably operate in concert with TG(C) in corneal wound healing.
Authors: Andri K Riau; Tina T Wong; Wanwen Lan; Sharon N Finger; Shyam S Chaurasia; Ai Hua Hou; Silin Chen; Shang Juan Yu; Louis Tong Journal: PLoS One Date: 2011-02-16 Impact factor: 3.240
Authors: Francisco Velez V; Jeffrey A Romano; Robert L McKown; Kari Green; Liwen Zhang; Ronald W Raab; Denise S Ryan; Cindy M L Hutnik; Henry F Frierson; Gordon W Laurie Journal: Invest Ophthalmol Vis Sci Date: 2013-03-01 Impact factor: 4.799
Authors: Lei Wang; Philipp C Uhlig; Eric F Eikenberry; Horst Robenek; Peter Bruckner; Uwe Hansen Journal: J Biol Chem Date: 2013-11-21 Impact factor: 5.157