PURPOSE: To study Mitomycin C Loaded Collagen Implant (CI) pharmacokinetics behaviour in vitro. METHODS: The CI were incubated for 15 minutes in different MMC loading solutions with the following concentrations: 0.03 mg/mL (n = 9), 0.3 mg/mL (n = 10) and 3.0 mg/mL (n = 10). The loaded CI were transferred in 100 micro L of 0.9% NaCl. Aqueous flow of 5 micro L/min was simulated. The MMC concentrations of the samples were determined by high performance liquid chromatography (HPLC). Dissolution kinetics were evaluated by a first-order process. The half-life of dissolution and the time of 95% dissolution were determined. RESULTS: The CI absorbed on average a MMC dose of 0.054, 0.530 and 6.090 micro g when incubated in the different MMC loading solutions containing 0.03 mg/mL, 0.3 mg/mL, 3.0 mg/mL of MMC, respectively. In the release experiments, the mean total dose delivered by CI was 0.0493, 0.585 and 5.291 micro g. A linear correlation between loading concentration and the estimated total dose released was demonstrated. The kinetic parameters showed a fast MMC dissolution. The half-life of the 3 series was 8.8, 10.1 and 10.5 min. CONCLUSIONS: Commercially available CI can be loaded with MMC, and could provide relatively slower release than sponge delivery of MMC. Clinical implications of these results warrants further studies.
PURPOSE: To study Mitomycin C Loaded Collagen Implant (CI) pharmacokinetics behaviour in vitro. METHODS: The CI were incubated for 15 minutes in different MMC loading solutions with the following concentrations: 0.03 mg/mL (n = 9), 0.3 mg/mL (n = 10) and 3.0 mg/mL (n = 10). The loaded CI were transferred in 100 micro L of 0.9% NaCl. Aqueous flow of 5 micro L/min was simulated. The MMC concentrations of the samples were determined by high performance liquid chromatography (HPLC). Dissolution kinetics were evaluated by a first-order process. The half-life of dissolution and the time of 95% dissolution were determined. RESULTS: The CI absorbed on average a MMC dose of 0.054, 0.530 and 6.090 micro g when incubated in the different MMC loading solutions containing 0.03 mg/mL, 0.3 mg/mL, 3.0 mg/mL of MMC, respectively. In the release experiments, the mean total dose delivered by CI was 0.0493, 0.585 and 5.291 micro g. A linear correlation between loading concentration and the estimated total dose released was demonstrated. The kinetic parameters showed a fast MMC dissolution. The half-life of the 3 series was 8.8, 10.1 and 10.5 min. CONCLUSIONS: Commercially available CI can be loaded with MMC, and could provide relatively slower release than sponge delivery of MMC. Clinical implications of these results warrants further studies.