Literature DB >> 14704668

Restriction enzyme-generated siRNA (REGS) vectors and libraries.

George Sen1, Tom S Wehrman, Jason W Myers, Helen M Blau.   

Abstract

Small interfering RNA (siRNA) technology facilitates the study of loss of gene function in mammalian cells and animal models, but generating multiple siRNA vectors using oligonucleotides is slow, inefficient and costly. Here we describe a new, enzyme-mediated method for generating numerous functional siRNA constructs from any gene of interest or pool of genes. To test our restriction enzyme-generated siRNA (REGS) system, we silenced a transgene and two endogenous genes and obtained the predicted phenotypes. REGS generated on average 34 unique siRNAs per kilobase of sequence. REGS enabled us to create enzymatically a complex siRNA library (>4 x 10(5) clones) from double-stranded cDNA encompassing known and unknown genes with 96% of the clones containing inserts of the appropriate size.

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Year:  2004        PMID： 14704668     DOI： 10.1038/ng1288

Source DB:  PubMed          Journal:  Nat Genet        ISSN： 1061-4036            Impact factor:   38.330


  38 in total

1.  A novel method to convert a DNA fragment inserted into a plasmid to an inverted repeat structure.

Authors:  Kazuya Tomimoto; Kosuke Fujita; Jun Ishibashi; Shigeo Imanishi; Minoru Yamakawa; Hiromitsu Tanaka
Journal:  Mol Biotechnol       Date:  2012-01       Impact factor: 2.695

Review 2.  Silence of the genes.

Authors:  Oded Singer; Amiel Yanai; Inder M Verma
Journal:  Proc Natl Acad Sci U S A       Date:  2004-04-05       Impact factor: 11.205

3.  A novel EST-derived RNAi screen reveals a critical role for farnesyl diphosphate synthase in β2-adrenergic receptor internalization and down-regulation.

Authors:  Xiaofeng Jiang; Hui Pan; Joseph F Nabhan; Ramaswamy Krishnan; Cynthia Koziol-White; Reynold A Panettieri; Quan Lu
Journal:  FASEB J       Date:  2012-01-25       Impact factor: 5.191

4.  A universal plasmid library encoding all permutations of small interfering RNA.

Authors:  Meihong Chen; Lishu Zhang; Hong-Yan Zhang; Xiahui Xiong; Bo Wang; Quan Du; Bing Lu; Claes Wahlestedt; Zicai Liang
Journal:  Proc Natl Acad Sci U S A       Date:  2005-02-04       Impact factor: 11.205

5.  Size unbiased representative enzymatically generated RNAi (SURER) library and application for RNAi therapeutic screens.

Authors:  Tiejun Li; York Yuanyuan Zhu; Li Chen; Yuncheng Sun; Jian Yuan; Michael Graham; Peter French
Journal:  Nucleic Acid Ther       Date:  2014-12-10       Impact factor: 5.486

6.  Inhibitors of MyD88-dependent proinflammatory cytokine production identified utilizing a novel RNA interference screening approach.

Authors:  John S Cho; Yun C Kim; Sherie L Morrison
Journal:  PLoS One       Date:  2009-09-15       Impact factor: 3.240

7.  Mouse Germ Cell Development in-vivo and in-vitro.

Authors:  Deshira Saiti; Orly Lacham-Kaplan
Journal:  Biomark Insights       Date:  2007-06-06

8.  Selection of potent non-toxic inhibitory sequences from a randomized HIV-1 specific lentiviral short hairpin RNA library.

Authors:  Carola Pongratz; Benjamin Yazdanpanah; Hamid Kashkar; Maik J Lehmann; Hans-Georg Kräusslich; Martin Krönke
Journal:  PLoS One       Date:  2010-10-08       Impact factor: 3.240

9.  Vector-based siRNA delivery strategies for high-throughput screening of novel target genes.

Authors:  Meihong Chen; Quan Du; Hong-Yan Zhang; Claes Wahlestedt; Zicai Liang
Journal:  J RNAi Gene Silencing       Date:  2005-07-27

10.  RNA modulators of complex phenotypes in mammalian cells.

Authors:  Angela Lai; Murray J Cairns; Nham Tran; Hong-Ping Zhang; Lara Cullen; Greg M Arndt
Journal:  PLoS One       Date:  2009-03-09       Impact factor: 3.240
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