| Literature DB >> 14700627 |
Oleg Kurnasov1, Vasiliy Goral, Keri Colabroy, Svetlana Gerdes, Shubha Anantha, Andrei Osterman, Tadhg P Begley.
Abstract
Previous studies have demonstrated two different biosynthetic pathways to quinolinate, the universal de novo precursor to the pyridine ring of NAD. In prokaryotes, quinolinate is formed from aspartate and dihydroxyacetone phosphate; in eukaryotes, it is formed from tryptophan. It has been generally believed that the tryptophan to quinolinic acid biosynthetic pathway is unique to eukaryotes; however, this paper describes the use of comparative genome analysis to identify likely candidates for all five genes involved in the tryptophan to quinolinic acid pathway in several bacteria. Representative examples of each of these genes were overexpressed, and the predicted functions are confirmed in each case using unambiguous biochemical assays.Entities:
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Year: 2003 PMID： 14700627 DOI： 10.1016/j.chembiol.2003.11.011
Source DB: PubMed Journal: Chem Biol ISSN： 1074-5521