R G Cayabyab1, C A Jones, K Y C Kwong, C Hendershott, C Lecart, P Minoo, R Ramanathan. 1. Division of Neonatology and Allergy/Immunology, Department of Pediatrics, Women's and Children's Hospital, LAC and USC Medical Center, University of Southern California School of Medicine, Los Angeles, California 90033, USA.
Abstract
OBJECTIVE: To determine whether the presence of the proinflammatory cytokine interleukin (IL)-1beta in the lungs of preterm infants immediately after birth was associated with maternal inflammation and could predict adverse neonatal outcome. STUDY DESIGN: Prospective evaluation of serially obtained tracheal aspirates for the presence of IL-1beta in 25 preterm infants (birth weight 595-1700 g; gestational age 24-32 weeks) with respiratory distress syndrome. The initial tracheal aspirate was obtained within 1 h after delivery. RESULTS: An initial tracheal aspirate positive for IL-1beta had a highly significant correlation with documented maternal chorioamnionitis for the given patient. In addition, the presence of IL-1beta correlated significantly with elevated total cell count (2.62 vs. 0.96 x 10(6)/ml, p = 0.0097), granulocyte count (2.12 vs. 0.22 x 10(6)/ml, p = 0.001), macrophage count (0.28 vs. 0.01 x 10(6)/ml, p = 0.02) and the presence of proinflammatory cytokines IL-6, IL-8 and tumor necrosis factor (TNF)-alpha. Preterm neonates positive for IL-1beta in their initial sample were on prolonged assisted ventilation (38 vs. 16 days, p = 0.013) and oxygen supplementation (62 vs. 40.5 days, p = 0.0462) and required prolonged hospitalization (69 vs. 46 days, p = 0.0165). CONCLUSIONS: The concentration of IL-1beta in the initial tracheal aspirate obtained from the lungs of preterm infants within the first hour of life may serve as a marker of antenatal/perinatal inflammation, probably due to maternal chorioamnionitis, and could predict an adverse clinical course and short-term outcome.
OBJECTIVE: To determine whether the presence of the proinflammatory cytokine interleukin (IL)-1beta in the lungs of preterm infants immediately after birth was associated with maternal inflammation and could predict adverse neonatal outcome. STUDY DESIGN: Prospective evaluation of serially obtained tracheal aspirates for the presence of IL-1beta in 25 preterm infants (birth weight 595-1700 g; gestational age 24-32 weeks) with respiratory distress syndrome. The initial tracheal aspirate was obtained within 1 h after delivery. RESULTS: An initial tracheal aspirate positive for IL-1beta had a highly significant correlation with documented maternal chorioamnionitis for the given patient. In addition, the presence of IL-1beta correlated significantly with elevated total cell count (2.62 vs. 0.96 x 10(6)/ml, p = 0.0097), granulocyte count (2.12 vs. 0.22 x 10(6)/ml, p = 0.001), macrophage count (0.28 vs. 0.01 x 10(6)/ml, p = 0.02) and the presence of proinflammatory cytokines IL-6, IL-8 and tumor necrosis factor (TNF)-alpha. Preterm neonates positive for IL-1beta in their initial sample were on prolonged assisted ventilation (38 vs. 16 days, p = 0.013) and oxygen supplementation (62 vs. 40.5 days, p = 0.0462) and required prolonged hospitalization (69 vs. 46 days, p = 0.0165). CONCLUSIONS: The concentration of IL-1beta in the initial tracheal aspirate obtained from the lungs of preterm infants within the first hour of life may serve as a marker of antenatal/perinatal inflammation, probably due to maternal chorioamnionitis, and could predict an adverse clinical course and short-term outcome.
Authors: Brandie D Wagner; Marci K Sontag; J Kirk Harris; Joshua I Miller; Lindsey Morrow; Charles E Robertson; Mark J Stephens; Brenda B Poindexter; Steven H Abman; Peter M Mourani Journal: J Matern Fetal Neonatal Med Date: 2017-11-28
Authors: Marcel F Nold; Niamh E Mangan; Ina Rudloff; Steven X Cho; Nikeh Shariatian; Thilini D Samarasinghe; Elizabeth M Skuza; John Pedersen; Alex Veldman; Philip J Berger; Claudia A Nold-Petry Journal: Proc Natl Acad Sci U S A Date: 2013-08-14 Impact factor: 11.205
Authors: Christine B Bui; Magdalena Kolodziej; Emma Lamanna; Kirstin Elgass; Arvind Sehgal; Ina Rudloff; Daryl O Schwenke; Hirotsugu Tsuchimochi; Maurice A G M Kroon; Steven X Cho; Anton Maksimenko; Marian Cholewa; Philip J Berger; Morag J Young; Jane E Bourke; James T Pearson; Marcel F Nold; Claudia A Nold-Petry Journal: Front Immunol Date: 2019-07-11 Impact factor: 7.561
Authors: Katheryn E Nathe; Christy J Mancuso; Richard Parad; Linda J Van Marter; Camilia R Martin; Liat Stoler-Barak; Victoria J Philbin; Michele F Phillips; Christine D Palmer; Ofer Levy Journal: Pediatr Res Date: 2012-08 Impact factor: 3.756
Authors: Ina Rudloff; Steven X Cho; Christine B Bui; Catriona McLean; Alex Veldman; Philip J Berger; Marcel F Nold; Claudia A Nold-Petry Journal: J Cell Mol Med Date: 2016-12-13 Impact factor: 5.310