OBJECTIVE: To study DNA damage of three kinds of gasoline oxygenates. METHOD: Single cell gel electrophoresis assay(Comet assay) was used to detect the damage effects of three gasoline oxygenates[methyl tertiary butyl ether(MTBE), ethanol anhydrous(EA) and dimethyl carbonate(DMC)] on DNA in L-929 mice fibroblasts. RESULTS: In certain concentation(37.500-150.000 mg/ml), MTBE could directly cause DNA damage of L-929 mice fibroblasts. There was obvious dose-effect relationship, i.e. when the concentration of MTBE was increased from 9.375 to 150.000 mg/ml, the comet rate also increased from 4% to 85%, and the length of comet tail changed correspondingly. The results of EA and DMC were negative. CONCLUSION: Under the condition of this experiment(150.000 mg/ml), MTBE could directly cause DNA damage while the effect of EA and DMC on DNA damage was not found.
OBJECTIVE: To study DNA damage of three kinds of gasoline oxygenates. METHOD: Single cell gel electrophoresis assay(Comet assay) was used to detect the damage effects of three gasoline oxygenates[methyl tertiary butyl ether(MTBE), ethanol anhydrous(EA) and dimethyl carbonate(DMC)] on DNA in L-929 mice fibroblasts. RESULTS: In certain concentation(37.500-150.000 mg/ml), MTBE could directly cause DNA damage of L-929 mice fibroblasts. There was obvious dose-effect relationship, i.e. when the concentration of MTBE was increased from 9.375 to 150.000 mg/ml, the comet rate also increased from 4% to 85%, and the length of comet tail changed correspondingly. The results of EA and DMC were negative. CONCLUSION: Under the condition of this experiment(150.000 mg/ml), MTBE could directly cause DNA damage while the effect of EA and DMC on DNA damage was not found.
Authors: Ahmad Salimi; Mehrdad Vaghar-Moussavi; Enayatollah Seydi; Jalal Pourahmad Journal: Environ Sci Pollut Res Int Date: 2016-01-22 Impact factor: 4.223
Authors: Stacey E Anderson; Jennifer Franko; Katie L Anderson; Albert E Munson; Ewa Lukomska; B Jean Meade Journal: J Immunotoxicol Date: 2012-09-07 Impact factor: 3.000