OBJECTIVE: To observe effects of beta-asaron on gene expression in BALB/c mouse brain by gene chip. METHODS: The mRNA of mouse brain samples of experiment group and control group was markered with cy5, cy3 fluorescence separately, and hybridized with 4000 genes on MGEC-40S gene chip after mix. Fluorescence intensity was scanned with Genepix 400 B scanner and analysed with Genepix Pro3.0 soft. RESULTS: Among 4000 genes, there are 15 genes with difference expression (the ratio > 1.9 or < 0.59) in two groups. In beta-asaron group, 4 genes expression raised relating to ion channels, substance transform through membrane, calcium-dependent protein kinase regulation, apoptosis, 11 genes expression declined relating to excitatory neurotransmitter metabolism in the CNS, T lymphocyte tend, genes expression regulation and drug metabolism. CONCLUSION: beta-asaron has effects on multi-target genes in mouse brain.
OBJECTIVE: To observe effects of beta-asaron on gene expression in BALB/c mouse brain by gene chip. METHODS: The mRNA of mouse brain samples of experiment group and control group was markered with cy5, cy3 fluorescence separately, and hybridized with 4000 genes on MGEC-40S gene chip after mix. Fluorescence intensity was scanned with Genepix 400 B scanner and analysed with Genepix Pro3.0 soft. RESULTS: Among 4000 genes, there are 15 genes with difference expression (the ratio > 1.9 or < 0.59) in two groups. In beta-asaron group, 4 genes expression raised relating to ion channels, substance transform through membrane, calcium-dependent protein kinase regulation, apoptosis, 11 genes expression declined relating to excitatory neurotransmitter metabolism in the CNS, T lymphocyte tend, genes expression regulation and drug metabolism. CONCLUSION:beta-asaron has effects on multi-target genes in mouse brain.