| Literature DB >> 14691111 |
K Tanimoto1, A Suzuki, S Ohno, K Honda, N Tanaka, T Doi, K Yoneno, M Ohno-Nakahara, Y Nakatani, M Ueki, K Tanne.
Abstract
Hyaluronan (HA) synthesis in the synovial membrane is affected by various chemical mediators. It is hypothesized that transforming growth factor-beta 1 (TGF-beta 1) would be a mediator to modulate HA synthesis in cultured synovial membrane fibroblasts of the temporomandibular joint (TMJ). Fibroblasts were extracted from the TMJ synovial membrane of four-week-old Japanese white rabbits. The amount of HA and expression levels of HA synthase (HAS) mRNAs induced by TGF-beta 1 treatment were analyzed by means of high-performance liquid chromatography and real-time polymerase chain-reaction, respectively. Both medium and large amounts of HA were enhanced by the stimulation of TGF-beta 1. HAS2 mRNA expression was enhanced 13-fold after six-hour stimulation with TGF-beta 1 (10 ng/mL), whereas HAS3 mRNA expression was not changed significantly. These results suggest that TGF-beta 1 enhances the expression of HAS2 mRNA in the TMJ synovial membrane fibroblasts and may contribute to the production of high-molecular-weight HA in the joint fluid.Entities:
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Year: 2004 PMID： 14691111 DOI： 10.1177/154405910408300108
Source DB: PubMed Journal: J Dent Res ISSN： 0022-0345 Impact factor: 6.116