Literature DB >> 14690634

Fluorescence readouts in HTS: no gain without pain?

Philip Gribbon1, Andreas Sewing.   

Abstract

Fluorescence-based detection technologies are frequently applied in biological testing, due to their unique advantages in setting up homogeneous, sensitive assays in miniaturized formats. However, the wide application of these readouts has highlighted challenges in reagent design and problems with interference from biological reagents and compounds. Here, we summarize the current application of fluorescence-based detection methodologies, focusing on the problems faced by assay developers and on solutions to reduce false positive and negative results in fluorescence-based HTS.

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Year:  2003        PMID： 14690634     DOI： 10.1016/s1359-6446(03)02895-2

Source DB:  PubMed          Journal:  Drug Discov Today        ISSN： 1359-6446            Impact factor:   7.851


  23 in total

1.  Distinct regulation of cytoplasmic calcium signals and cell death pathways by different plasma membrane calcium ATPase isoforms in MDA-MB-231 breast cancer cells.

Authors:  Merril C Curry; Nicole A Luk; Paraic A Kenny; Sarah J Roberts-Thomson; Gregory R Monteith
Journal:  J Biol Chem       Date:  2012-06-25       Impact factor: 5.157

2.  Post-HTS case report and structural alert: Promiscuous 4-aroyl-1,5-disubstituted-3-hydroxy-2H-pyrrol-2-one actives verified by ALARM NMR.

Authors:  Jayme L Dahlin; J Willem M Nissink; Subhashree Francis; Jessica M Strasser; Kristen John; Zhiguo Zhang; Michael A Walters
Journal:  Bioorg Med Chem Lett       Date:  2015-08-10       Impact factor: 2.823

3.  An adaptable luminescence resonance energy transfer assay for measuring and screening protein-protein interactions and their inhibition.

Authors:  Engin Yapici; D Rajasekhar Reddy; Lawrence W Miller
Journal:  Chembiochem       Date:  2012-01-23       Impact factor: 3.164

4.  Calculation of photon-count number distributions via master equations.

Authors:  Kaupo Palo; Ulo Mets; Vello Loorits; Peet Kask
Journal:  Biophys J       Date:  2005-12-30       Impact factor: 4.033

5.  Resonant waveguide grating biosensor for living cell sensing.

Authors:  Ye Fang; Ann M Ferrie; Norman H Fontaine; John Mauro; Jitendra Balakrishnan
Journal:  Biophys J       Date:  2006-06-09       Impact factor: 4.033

Review 6.  Insights into GPCR pharmacology from the measurement of changes in intracellular cyclic AMP; advantages and pitfalls of differing methodologies.

Authors:  Stephen J Hill; Christine Williams; Lauren T May
Journal:  Br J Pharmacol       Date:  2010-11       Impact factor: 8.739

7.  Measuring and interpreting the selectivity of protein kinase inhibitors.

Authors:  Lynette A Smyth; Ian Collins
Journal:  J Chem Biol       Date:  2009-06-06

8.  A general method for discovering inhibitors of protein-DNA interactions using photonic crystal biosensors.

Authors:  Leo L Chan; Maria Pineda; James T Heeres; Paul J Hergenrother; Brian T Cunningham
Journal:  ACS Chem Biol       Date:  2008-07-18       Impact factor: 5.100

9.  Fluorescence lifetime plate reader: resolution and precision meet high-throughput.

Authors:  Karl J Petersen; Kurt C Peterson; Joseph M Muretta; Sutton E Higgins; Gregory D Gillispie; David D Thomas
Journal:  Rev Sci Instrum       Date:  2014-11       Impact factor: 1.523

10.  Plate-based diversity subset screening: an efficient paradigm for high throughput screening of a large screening file.

Authors:  Andrew S Bell; Joseph Bradley; Jeremy R Everett; Michelle Knight; Jens Loesel; John Mathias; David McLoughlin; James Mills; Robert E Sharp; Christine Williams; Terence P Wood
Journal:  Mol Divers       Date:  2013-04-05       Impact factor: 2.943
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