Literature DB >> 14688228

Role of the zinc fingers of HIV-1 nucleocapsid protein in maturation of genomic RNA.

Seiki Baba1, Ken-Ichi Takahashi, Yoshio Koyanagi, Naoki Yamamoto, Hiroshi Takaku, Robert J Gorelick, Gota Kawai.   

Abstract

The nucleocapsid protein of HIV-1 consists of two basic amino acid regions and two zinc fingers. We investigated the requirement of these domains for the structural conversion of a 39mer RNA covering the dimerization initiation site by using three peptides; wild-type NCp7, a mutant in which the two zinc fingers are mutated, and another mutant in which the two zinc fingers are deleted. The two mutants exhibited similar conversion activities to each other, which were lower than that of the wild-type, indicating that the two basic regions exhibit some activity for RNA chaperone, as we suggested before, and the zinc fingers enhance the efficiency of this activity.

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Year:  2003        PMID： 14688228     DOI： 10.1093/jb/mvg200

Source DB:  PubMed          Journal:  J Biochem        ISSN： 0021-924X            Impact factor:   3.387


  9 in total

1.  Post-translational intracellular trafficking determines the type of immune response elicited by DNA vaccines expressing Gag antigen of Human Immunodeficiency Virus Type 1 (HIV-1).

Authors:  Aaron Wallace; Kim West; Alan L Rothman; Francis A Ennis; Shan Lu; Shixia Wang
Journal:  Hum Vaccin Immunother       Date:  2013-08-13       Impact factor: 3.452

2.  Structure/function mapping of amino acids in the N-terminal zinc finger of the human immunodeficiency virus type 1 nucleocapsid protein: residues responsible for nucleic acid helix destabilizing activity.

Authors:  Nirupama Narayanan; Robert J Gorelick; Jeffrey J DeStefano
Journal:  Biochemistry       Date:  2006-10-17       Impact factor: 3.162

3.  Stability and conformation of the dimeric HIV-1 genomic RNA 5'UTR.

Authors:  Robert J Blakemore; Cleo Burnett; Canessa Swanson; Siarhei Kharytonchyk; Alice Telesnitsky; James B Munro
Journal:  Biophys J       Date:  2021-09-14       Impact factor: 4.033

4.  A new role for HIV nucleocapsid protein in modulating the specificity of plus strand priming.

Authors:  Deena T Jacob; Jeffrey J DeStefano
Journal:  Virology       Date:  2008-07-15       Impact factor: 3.616

5.  The N-terminal zinc finger and flanking basic domains represent the minimal region of the human immunodeficiency virus type-1 nucleocapsid protein for targeting chaperone function.

Authors:  Mithun Mitra; Wei Wang; My-Nuong Vo; Ioulia Rouzina; George Barany; Karin Musier-Forsyth
Journal:  Biochemistry       Date:  2013-11-06       Impact factor: 3.162

6.  Effect of Mg(2+) and Na(+) on the nucleic acid chaperone activity of HIV-1 nucleocapsid protein: implications for reverse transcription.

Authors:  My-Nuong Vo; George Barany; Ioulia Rouzina; Karin Musier-Forsyth
Journal:  J Mol Biol       Date:  2009-01-06       Impact factor: 5.469

7.  Molecular determinants of HIV-1 NCp7 chaperone activity in maturation of the HIV-1 dimerization initiation site.

Authors:  Raviprasad Aduri; Katharine T Briggs; Robert J Gorelick; John P Marino
Journal:  Nucleic Acids Res       Date:  2012-12-28       Impact factor: 16.971

Review 8.  Interaction of viral proteins with metal ions: role in maintaining the structure and functions of viruses.

Authors:  Umesh C Chaturvedi; Richa Shrivastava
Journal:  FEMS Immunol Med Microbiol       Date:  2005-02-01

9.  RNA-Binding Domains of Heterologous Viral Proteins Substituted for Basic Residues in the RSV Gag NC Domain Restore Specific Packaging of Genomic RNA.

Authors:  Breanna L Rice; Timothy L Lochmann; Leslie J Parent
Journal:  Viruses       Date:  2020-03-27       Impact factor: 5.048
  9 in total

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