R G K Leuschner1, N Beresford-Jones, C Robinson. 1. Department for Environment, Food and Rural Affairs, Central Science Laboratory, Sand Hutton, York, UK. r.leuschner@csl.gov.uk
Abstract
AIM: Application of MALDI-TOF MS for characterization of strains of Salmonella enterica subsp. enterica. METHODS AND RESULTS: Whole cells were analysed by MALDI-TOF MS. Spectra with a maximum of 500 mass peaks between (m/z) 0 and 25000 were examined for consensus peaks manually and by a computer software algorithm. Consensus peaks were observed by both methods for spectra of Salmonella enterica serovars Derby, Hadar, Virchow, Anatum, Typhimurium and Enteritidis. CONCLUSIONS: Differences in numbers of consensus peaks in spectra obtained by manual and computer comparison indicated that development of the software involving statistical analysis of peak accuracy is necessary. SIGNIFICANCE AND IMPACT OF THE STUDY: Development of an analysis system for peak profiles in whole cell MALDI-TOF MS spectra to enable intra and interlaboratory comparison.
AIM: Application of MALDI-TOF MS for characterization of strains of Salmonella enterica subsp. enterica. METHODS AND RESULTS: Whole cells were analysed by MALDI-TOF MS. Spectra with a maximum of 500 mass peaks between (m/z) 0 and 25000 were examined for consensus peaks manually and by a computer software algorithm. Consensus peaks were observed by both methods for spectra of Salmonella enterica serovars Derby, Hadar, Virchow, Anatum, Typhimurium and Enteritidis. CONCLUSIONS: Differences in numbers of consensus peaks in spectra obtained by manual and computer comparison indicated that development of the software involving statistical analysis of peak accuracy is necessary. SIGNIFICANCE AND IMPACT OF THE STUDY: Development of an analysis system for peak profiles in whole cell MALDI-TOF MS spectra to enable intra and interlaboratory comparison.
Authors: Elena N Ilina; Alexandra D Borovskaya; Maja M Malakhova; Vladimir A Vereshchagin; Anna A Kubanova; Alexander N Kruglov; Tatyana S Svistunova; Anaida O Gazarian; Thomas Maier; Markus Kostrzewa; Vadim M Govorun Journal: J Mol Diagn Date: 2008-12-18 Impact factor: 5.568
Authors: Sukhadeo B Barbuddhe; Thomas Maier; Gerold Schwarz; Markus Kostrzewa; Herbert Hof; Eugen Domann; Trinad Chakraborty; Torsten Hain Journal: Appl Environ Microbiol Date: 2008-07-07 Impact factor: 4.792
Authors: Anna Rettinger; Inke Krupka; Karola Grünwald; Viktor Dyachenko; Volker Fingerle; Regina Konrad; Heribert Raschel; Ulrich Busch; Andreas Sing; Reinhard K Straubinger; Ingrid Huber Journal: BMC Microbiol Date: 2012-08-27 Impact factor: 3.605
Authors: Rebecca L Bell; Karen G Jarvis; Andrea R Ottesen; Melinda A McFarland; Eric W Brown Journal: Microb Biotechnol Date: 2016-04-04 Impact factor: 5.813
Authors: Matthew L Faron; Blake W Buchan; Josh Hyke; Neil Madisen; Jennifer L Lillie; Paul A Granato; Deborah A Wilson; Gary W Procop; Susan Novak-Weekley; Elizabeth Marlowe; Joven Cumpio; Christen Griego-Fullbright; Sandra Kindig; Karen Timm; Stephen Young; Nathan A Ledeboer Journal: PLoS One Date: 2015-11-03 Impact factor: 3.240