Literature DB >> 1468428

Improved detection of HIV p24 antigen in serum after acid pretreatment.

M A Rodríguez-Iglesias1, J R Alvarez, A Vergara, M S Garcia-Valdivia, I Jesús, J Mira.   

Abstract

HIV-1 p24 antigen was detected in 554 sera (509 from HIV-1 seropositive individuals and 45 sera from seronegative controls) using a conventional method with acid pretreatment of the sample in order to separate the p24 antigen/anti-p24 antibody immune complexes. In asymptomatic individuals there was a substantial increase in antigen detection (48.2% vs 8.4%). Similar results were also observed in ARC (59.1% vs 12.2%) and AIDS patients (85.7% vs 37.1%). It can be concluded that the acid treatment improves the sensitivity of conventional techniques to detect HIV-1 p24 antigen.

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Year:  1992        PMID： 1468428     DOI： 10.1007/bf01960890

Source DB:  PubMed          Journal:  Eur J Clin Microbiol Infect Dis        ISSN： 0934-9723            Impact factor:   3.267


  7 in total

1.  A simple method for improved assay demonstrates that HIV p24 antigen is present as immune complexes in most sera from HIV-infected individuals.

Authors:  P Nishanian; K R Huskins; S Stehn; R Detels; J L Fahey
Journal:  J Infect Dis       Date:  1990-07       Impact factor: 5.226

2.  Significant changes in HIV antigen level in the serum of patients treated with azidothymidine.

Authors:  R E Chaisson; J P Allain; M Leuther; P A Volberding
Journal:  N Engl J Med       Date:  1986-12-18       Impact factor: 91.245

3.  Antigenemia and antibody titers to core and envelope antigens in AIDS, AIDS-related complex, and subclinical human immunodeficiency virus infection.

Authors:  J Goudsmit; J M Lange; D A Paul; G J Dawson
Journal:  J Infect Dis       Date:  1987-03       Impact factor: 5.226

4.  Acid dissociation increases the sensitivity of p24 antigen detection for the evaluation of antiviral therapy and disease progression in asymptomatic human immunodeficiency virus-infected persons.

Authors:  R C Bollinger; R L Kline; H L Francis; M W Moss; J G Bartlett; T C Quinn
Journal:  J Infect Dis       Date:  1992-05       Impact factor: 5.226

5.  Pathogenesis of HIV and its implications for serodiagnosis and monitoring of antiviral therapy.

Authors:  J Goudsmit; J M Lange; W J Krone; M B Teunissen; L G Epstein; S A Danner; H van den Berg; C Breederveld; L Smit; M Bakker
Journal:  J Virol Methods       Date:  1987-08       Impact factor: 2.014

6.  Correlation of serum HIV antigen and antibody with clinical status in HIV-infected patients.

Authors:  D A Paul; L A Falk; H A Kessler; R M Chase; B Blaauw; D S Chudwin; A L Landay
Journal:  J Med Virol       Date:  1987-08       Impact factor: 2.327

7.  Effect of zidovudine on serum human immunodeficiency virus antigen levels in symptom-free subjects.

Authors:  F de Wolf; J M Lange; J Goudsmit; P Cload; J de Gans; P T Schellekens; R A Coutinho; A P Fiddian; J van der Noordaa
Journal:  Lancet       Date:  1988-02-20       Impact factor: 79.321
  7 in total
  1 in total

1.  Measurement of human immunodeficiency virus type 1 p24 in serum by an ultrasensitive enzyme immunoassay, the two-site immune complex transfer enzyme immunoassay.

Authors:  S Hashida; K Hashinaka; I Nishikata; S Oka; K Shimada; A Saitoh; A Takamizawa; H Shinagawa; E Ishikawa
Journal:  J Clin Microbiol       Date:  1995-02       Impact factor: 5.948
  1 in total

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