Literature DB >> 146824

Purification and properties of adenosine triphosphatase solubilized from beef heart mitochondria by chloroform.

J Kopecký, J Houstĕk, Z Drahota.   

Abstract

Soluble, oligomycin-insensitive ATPase released from beef heart mitchondria by chloroform extraction can be further purified by Sepharose 6B gel filtration. This purification increases enzyme activity 4--5 times (100-130U/mg). According to specific activity, high purity and ability to reconstitute oligomycin-sensitive complex, isolated ATPase is quite comparable with enzyme preparations isolated by other methods.

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Year:  1977        PMID： 146824     DOI： 10.1007/bf00280271

Source DB:  PubMed          Journal:  Mol Cell Biochem        ISSN： 0300-8177            Impact factor:   3.396


  10 in total

1.  A simple and rapid method for the preparation of adenosine triphosphatase from submitochondrial particles.

Authors:  R B Beechey; S A Hubbard; P E Linnett; A D Mitchell; E A Munn
Journal:  Biochem J       Date:  1975-06       Impact factor: 3.857

2.  A simple procedure for isolating adenosine triphosphatase from mitochondria.

Authors:  Z Drahota; J Houstĕk
Journal:  Biochim Biophys Acta       Date:  1977-06-09

3.  A NATURALLY OCCURRING INHIBITOR OF MITOCHONDRIAL ADENOSINE TRIPHOSPHATASE.

Authors:  M E PULLMAN; G C MONROY
Journal:  J Biol Chem       Date:  1963-11       Impact factor: 5.157

4.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

5.  Studies on the mitochondrial adenosine triphosphatase system. I. Restoration of adenosine triphosphate-dependent reactions in salt-extracted submitochondrial particles.

Authors:  D H MacLennan; J M Smoly; A Tzagoloff
Journal:  J Biol Chem       Date:  1968-04-10       Impact factor: 5.157

6.  Partial resolution of the enzyme catalyzing oxidative phosphorylation. XXII. Interaction between mitochondrial adenosine triphosphatase inhibitor and mitochondrial adenosine triphosphatase.

Authors:  L L Horstman; E Racker
Journal:  J Biol Chem       Date:  1970-03-25       Impact factor: 5.157

7.  Studies on the mitochondrial oligomycin-insensitivt ATPase. I. An improved method of purification and the behavior of the enzyme in solutions of various depolymerizing agents.

Authors:  A E Senior; J C Brooks
Journal:  Arch Biochem Biophys       Date:  1970-09       Impact factor: 4.013

8.  The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.

Authors:  K Weber; M Osborn
Journal:  J Biol Chem       Date:  1969-08-25       Impact factor: 5.157

9.  Effects of triphenylsulphonium ions on mitochondria. Inhibition of adenosine triphosphatase activity.

Authors:  R H Barrett; M J Selwyn
Journal:  Biochem J       Date:  1976-05-15       Impact factor: 3.857

10.  Mitochondrial adenosine triphosphatase from yeast, Saccharomyces cerevisiae. Purification, subunit structure and kinetics.

Authors:  K Takeshige; B Hess; M Böhm; H Zimmermann-Telschow
Journal:  Hoppe Seylers Z Physiol Chem       Date:  1976-11
  10 in total
  2 in total

1.  Antimitochondrial antibodies (AMA) in primary biliary cirrhosis. I. Separation of the PBC antigen activity from mitochondrial ATPase activity.

Authors:  T Sayers; A Leoutsakos; P Berg; H Baum
Journal:  J Bioenerg Biomembr       Date:  1981-12       Impact factor: 2.945

2.  Mitochondrial antibodies in primary biliary cirrhosis. VI. Association of the complement fixing antigen with a component of the mitochondrial F1-ATPase complex.

Authors:  J Lindenborn-Fotinos; T J Sayers; P A Berg
Journal:  Clin Exp Immunol       Date:  1982-11       Impact factor: 4.330
  2 in total

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