Literature DB >> 1468141

Development and validation of a robust specific enzyme mediated assay for phenylalanine in serum.

R S Campbell1, G M Brearley, H Varsani, H C Morris, T P Milligan, S K Hall, P M Hammond, C P Price.   

Abstract

The specificity of a phenylalanine dehydrogenase, particularly with respect to cross reactivity toward tyrosine, has been shown to be pH dependent, being minimal at high pH. The dehydrogenase step has been coupled to colorimetric detection of NADH using a tetrazolium salt. The assay shows no significant cross reactivity towards a range of amino acids or drugs and correlates well with an established HPLC technique.

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Year:  1992        PMID: 1468141     DOI: 10.1016/0009-8981(92)90205-5

Source DB:  PubMed          Journal:  Clin Chim Acta        ISSN: 0009-8981            Impact factor:   3.786


  2 in total

1.  Towards self-monitoring and self-treatment in phenylketonuria--a way to better diet compliance.

Authors:  U Wendel; U Langenbeck
Journal:  Eur J Pediatr       Date:  1996-07       Impact factor: 3.183

2.  Development of a Whole Blood Paper-Based Device for Phenylalanine Detection in the Context of PKU Therapy Monitoring.

Authors:  Robert Robinson; Liam Wong; Raymond J Monnat; Elain Fu
Journal:  Micromachines (Basel)       Date:  2016-02-15       Impact factor: 2.891

  2 in total

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