Literature DB >> 14677891

A comprehensive comparison of Ziehl-Neelsen and fluorescence microscopy for the diagnosis of tuberculosis in a resource-poor urban setting.

L E A Kivihya-Ndugga1, M R A van Cleeff, W A Githui, L W Nganga, D K Kibuga, J A Odhiambo, Paul R Klatser.   

Abstract

SETTING: Nairobi City Council Chest Clinic, Kenya.
OBJECTIVES: To establish the efficiency, costs and cost-effectiveness of six diagnostic strategies using Ziehl-Neelsen (ZN) and fluorescence microscopy (FM).
DESIGN: A cross-sectional study of 1398 TB suspects attending a specialised chest clinic in Nairobi subjected to three sputum examinations by ZN and FM. Lowenstein-Jensen culture was used as the gold standard. Cost analysis included health service and patient costs.
RESULTS: Of 1398 suspects enrolled, 993 (71%) had a complete diagnostic work-up involving three sputum specimens for ZN and FM, culture and chest X-ray (CXR). Irrespective of whether ZN or FM was used on one, two or three smears, the overall diagnostic process detected 92% culture-positive cases. Different strategies affected the ratio of smear-positive to smear-negative TB; however, FM was more sensitive than ZN (P < 0.001). FM performance was not affected by the patient's HIV status. The cost per correctly diagnosed smear-positive case, including savings, was 40.30 US dollars for FM on two specimens compared to 57.70 US dollars for ZN on three specimens.
CONCLUSION: The FM method used on one or two specimens is more cost-effective and shortens the diagnostic process. Consequently, more patients can be put on a regimen for smear-positive TB, contributing to improved treatment and reducing transmission.

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Year:  2003        PMID: 14677891

Source DB:  PubMed          Journal:  Int J Tuberc Lung Dis        ISSN: 1027-3719            Impact factor:   2.373


  34 in total

1.  HIV-related incremental yield of bleach sputum concentration and fluorescence technique for the microscopic detection of tuberculosis.

Authors:  S I Eyangoh; G Torrea; M C Tejiokem; Y Kamdem; F F Piam; J Noeske; A Van Deun
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2008-05-08       Impact factor: 3.267

2.  Increased sensitivity in diagnosis of tuberculosis in HIV-positive patients through the small-membrane-filter method of microscopy.

Authors:  Patrícia Quincó; Samira Bührer-Sékula; Walber Brandão; Rossiclea Monte; Silvia Leopoldina Souza; Valeria Saraceni; Moises Palaci; Reynaldo Dietze; Marcelo Cordeiro-Santos
Journal:  J Clin Microbiol       Date:  2013-06-26       Impact factor: 5.948

3.  Comparative performance of urinary lipoarabinomannan assays and Xpert MTB/RIF in HIV-infected individuals.

Authors:  Maunank Shah; Willy Ssengooba; Derek Armstrong; Lydia Nakiyingi; Molly Holshouser; Jerrold J Ellner; Moses Joloba; Yukari C Manabe; Susan E Dorman
Journal:  AIDS       Date:  2014-06-01       Impact factor: 4.177

4.  Cost-effectiveness of novel algorithms for rapid diagnosis of tuberculosis in HIV-infected individuals in Uganda.

Authors:  Maunank Shah; David Dowdy; Moses Joloba; Willy Ssengooba; Yukari C Manabe; Jerrold Ellner; Susan E Dorman
Journal:  AIDS       Date:  2013-11-28       Impact factor: 4.177

Review 5.  Improving the diagnosis of tuberculosis: From QuantiFERON to new techniques to diagnose tuberculosis infections.

Authors:  Amy Y Vittor; Joseph M Garland; David Schlossberg
Journal:  Curr HIV/AIDS Rep       Date:  2011-09       Impact factor: 5.071

6.  Molecular detection and identification of mycobacterium tuberculosis complex and four clinically important nontuberculous mycobacterial species in smear-negative clinical samples by the genotype mycobacteria direct test.

Authors:  Can Bicmen; Ayriz T Gunduz; Meral Coskun; Gunes Senol; A Kadri Cirak; Ayse Ozsoz
Journal:  J Clin Microbiol       Date:  2011-06-08       Impact factor: 5.948

Review 7.  Novel developments in the epidemic of human immunodeficiency virus and tuberculosis coinfection.

Authors:  Asha Anandaiah; Keertan Dheda; Joseph Keane; Henry Koziel; David A J Moore; Naimish R Patel
Journal:  Am J Respir Crit Care Med       Date:  2010-12-22       Impact factor: 21.405

8.  Determining the burden of tuberculosis in Eritrea: a new approach.

Authors:  Mineab Sebhatu; Bahlbi Kiflom; Melles Seyoum; Nuredin Kassim; Tesfazion Negash; Andeberhan Tesfazion; Martien W Borgdorff; Marieke J van der Werf
Journal:  Bull World Health Organ       Date:  2007-08       Impact factor: 9.408

9.  Portable, battery-operated, low-cost, bright field and fluorescence microscope.

Authors:  Andrew R Miller; Gregory L Davis; Z Maria Oden; Mohamad Reza Razavi; Abolfazl Fateh; Morteza Ghazanfari; Farid Abdolrahimi; Shahin Poorazar; Fatemeh Sakhaie; Randall J Olsen; Ahmad Reza Bahrmand; Mark C Pierce; Edward A Graviss; Rebecca Richards-Kortum
Journal:  PLoS One       Date:  2010-08-04       Impact factor: 3.240

10.  Automated tuberculosis diagnosis using fluorescence images from a mobile microscope.

Authors:  Jeannette Chang; Pablo Arbeláez; Neil Switz; Clay Reber; Asa Tapley; J Lucian Davis; Adithya Cattamanchi; Daniel Fletcher; Jitendra Malik
Journal:  Med Image Comput Comput Assist Interv       Date:  2012
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