BACKGROUND AND OBJECTIVE: Most of the studies of laser-induced damage do not analyze individual cells. Objective of this work was to evaluate local photo-induced thermal phenomena in single cells at theoretical and experimental levels for developing the method for real-time detection of laser damage in intact cells. STUDY DESIGN/ MATERIALS AND METHODS: Theoretical model of cell-laser interaction assumes local nature of photo-induced thermal effects and describes photodamage through bubble formation. Photothermal (PT) method was suggested for damage detection. Laser-induced damage was verified for individual cells with two techniques through detection of Trypan blue penetration into damaged cell. RESULTS: Specific PT responses from blast-transformed lymphocytes were identified independently as result of bubble formation and cell damage. Probability of cell damage was obtained for cells as function of laser pulse energy. CONCLUSIONS: The Laser load test (LLT) was suggested for real-time detection of damage, damage threshold measurement, and investigation of intact single cells. Copyright 2003 Wiley-Liss, Inc.
BACKGROUND AND OBJECTIVE: Most of the studies of laser-induced damage do not analyze individual cells. Objective of this work was to evaluate local photo-induced thermal phenomena in single cells at theoretical and experimental levels for developing the method for real-time detection of laser damage in intact cells. STUDY DESIGN/ MATERIALS AND METHODS: Theoretical model of cell-laser interaction assumes local nature of photo-induced thermal effects and describes photodamage through bubble formation. Photothermal (PT) method was suggested for damage detection. Laser-induced damage was verified for individual cells with two techniques through detection of Trypan blue penetration into damaged cell. RESULTS: Specific PT responses from blast-transformed lymphocytes were identified independently as result of bubble formation and cell damage. Probability of cell damage was obtained for cells as function of laser pulse energy. CONCLUSIONS: The Laser load test (LLT) was suggested for real-time detection of damage, damage threshold measurement, and investigation of intact single cells. Copyright 2003 Wiley-Liss, Inc.
Authors: Francisco G Pérez-Gutiérrez; Santiago Camacho-López; Rodger Evans; Gabriel Guillén; Benjamin S Goldschmidt; John A Viator; Guillermo Aguilar Journal: Ann Biomed Eng Date: 2010-06-30 Impact factor: 3.934
Authors: Ekaterina Y Lukianova-Hleb; Alexander O Oginsky; John S Olson; Dmitri O Lapotko Journal: Lasers Surg Med Date: 2011-02-02 Impact factor: 4.025