OBJECTIVE: To study lipid fraction that is occasionally observed in red blood cell (RBC) hemolysate (supernatants from which membranes were separated). STUDY DESIGN: Plasma lipid profiles, cholesterol (Ch) and phospholipids (PL) in intact RBCs, RBC membranes and hemolysates were examined in young healthy male population in winter and summer. RESULTS: The RBC Ch and PL content was significantly higher than in membranes, both in winter and summer. The "excess" of cholesterol (associated with phospholipid) was bound to hemoglobin yielding Hb-lipid adduct (Hb-Ch), the pools in the RBC membrane remaining virtually unaltered. Levels of hemoglobin-lipid complex (Hb-Ch), which were significantly higher in winter than in summer (30% and 19% of the total Hb, respectively), positively correlated with plasma HDL cholesterol levels. CONCLUSION: To our knowledge, this is the first demonstration of cholesterol binding to Hb. The results suggest influence of plasma lipoprotein metabolism on the formation of Hb-Ch.
OBJECTIVE: To study lipid fraction that is occasionally observed in red blood cell (RBC) hemolysate (supernatants from which membranes were separated). STUDY DESIGN: Plasma lipid profiles, cholesterol (Ch) and phospholipids (PL) in intact RBCs, RBC membranes and hemolysates were examined in young healthy male population in winter and summer. RESULTS: The RBC Ch and PL content was significantly higher than in membranes, both in winter and summer. The "excess" of cholesterol (associated with phospholipid) was bound to hemoglobin yielding Hb-lipid adduct (Hb-Ch), the pools in the RBC membrane remaining virtually unaltered. Levels of hemoglobin-lipid complex (Hb-Ch), which were significantly higher in winter than in summer (30% and 19% of the total Hb, respectively), positively correlated with plasma HDL cholesterol levels. CONCLUSION: To our knowledge, this is the first demonstration of cholesterol binding to Hb. The results suggest influence of plasma lipoprotein metabolism on the formation of Hb-Ch.
Authors: Rgia A Othman; Semone B Myrie; David Mymin; Louise S Merkens; Jean-Baptiste Roullet; Robert D Steiner; Peter J H Jones Journal: J Pediatr Date: 2014-10-16 Impact factor: 4.406
Authors: Kimberly T Hung; Stela Z Berisha; Brian M Ritchey; Jennifer Santore; Jonathan D Smith Journal: Arterioscler Thromb Vasc Biol Date: 2012-04-12 Impact factor: 8.311
Authors: Solomon O Rotimi; David A Ojo; Olusola A Talabi; Regina N Ugbaja; Elizabeth A Balogun; Oladipo Ademuyiwa Journal: Lipids Health Dis Date: 2015-02-21 Impact factor: 3.876