BACKGROUND/AIMS: Several lines of experimental data suggest that acinar cells have the potential to differentiate into ductular-like cells, and these newly formed acinoductular cells may act as (facultative) stem cells. The purpose of this study was to test this hypothesis in a model of pancreatic regeneration in rats. METHODS: In the current study, using a 90% pancreatectomy as a rat model for pancreatic regeneration, we serially examined the pancreatic tissues in a time-dependent manner by conventional histology and immunostaining. Cell proliferation was assessed by in vivo bromodeoxyuridine (BrdU) labeling. RESULTS: By 24 h after surgery, acini showed depleted granules with more dilated lumens (acinar ectasia). By day 2, focal regions of regeneration appeared which were separated by fibrosis and composed of ductular-like cells (tubular complexes) and acinar cells with dilated lumina. Double immunofluorescent staining revealed that both amylase and CK19 were positive in the same cells localized to the focus of regeneration from the animals on day 2, the phenotype of cells from those regions apparently reverted to acinar cells, and the regions completely disappeared by day 7. By 48 h after surgery, the number of BrdU-positive cells increased 4.3-fold in ductular cells, and 2.5-fold in acinar cells compared with control tissue. CONCLUSION: Acinar cells through acinoductular metaplasia may be a source of pancreatic regeneration. Copyright 2003 S. Karger AG, Basel and IAP
BACKGROUND/AIMS: Several lines of experimental data suggest that acinar cells have the potential to differentiate into ductular-like cells, and these newly formed acinoductular cells may act as (facultative) stem cells. The purpose of this study was to test this hypothesis in a model of pancreatic regeneration in rats. METHODS: In the current study, using a 90% pancreatectomy as a rat model for pancreatic regeneration, we serially examined the pancreatic tissues in a time-dependent manner by conventional histology and immunostaining. Cell proliferation was assessed by in vivo bromodeoxyuridine (BrdU) labeling. RESULTS: By 24 h after surgery, acini showed depleted granules with more dilated lumens (acinar ectasia). By day 2, focal regions of regeneration appeared which were separated by fibrosis and composed of ductular-like cells (tubular complexes) and acinar cells with dilated lumina. Double immunofluorescent staining revealed that both amylase and CK19 were positive in the same cells localized to the focus of regeneration from the animals on day 2, the phenotype of cells from those regions apparently reverted to acinar cells, and the regions completely disappeared by day 7. By 48 h after surgery, the number of BrdU-positive cells increased 4.3-fold in ductular cells, and 2.5-fold in acinar cells compared with control tissue. CONCLUSION: Acinar cells through acinoductular metaplasia may be a source of pancreatic regeneration. Copyright 2003 S. Karger AG, Basel and IAP
Authors: Jennifer Kl Colby; Russell D Klein; Mark J McArthur; Claudio J Conti; Kaoru Kiguchi; Toru Kawamoto; Penny K Riggs; Amy I Pavone; Janet Sawicki; Susan M Fischer Journal: Neoplasia Date: 2008-08 Impact factor: 5.715
Authors: Oliver Strobel; Yuval Dor; Amy Stirman; Amanda Trainor; Carlos Fernández-del Castillo; Andrew L Warshaw; Sarah P Thayer Journal: Proc Natl Acad Sci U S A Date: 2007-03-07 Impact factor: 11.205
Authors: Oliver Strobel; Yuval Dor; Janivette Alsina; Amy Stirman; Gregory Lauwers; Amanda Trainor; Carlos Fernández-Del Castillo; Andrew L Warshaw; Sarah P Thayer Journal: Gastroenterology Date: 2007-09-14 Impact factor: 22.682