| Literature DB >> 14671332 |
Steven Vandenabeele1, Katrien Van Der Kelen, James Dat, Ilya Gadjev, Tom Boonefaes, Stijn Morsa, Pieter Rottiers, Luit Slooten, Marc Van Montagu, Marc Zabeau, Dirk Inze, Frank Van Breusegem.
Abstract
Hydrogen peroxide plays a central role in launching the defense response during stress in plants. To establish a molecular profile provoked by a sustained increase in hydrogen peroxide levels, catalase-deficient tobacco plants (CAT1AS) were exposed to high light (HL) intensities over a detailed time course. The expression kinetics of >14000 genes were monitored by using transcript profiling technology based on cDNA-amplified fragment length polymorphism. Clustering and sequence analysis of 713 differentially expressed transcript fragments revealed a transcriptional response that mimicked that reported during both biotic and abiotic stresses, including the up-regulation of genes involved in the hypersensitive response, vesicular transport, posttranscriptional processes, biosynthesis of ethylene and jasmonic acid, proteolysis, mitochondrial metabolism, and cell death, and was accompanied by a very rapid up-regulation of several signal transduction components. Expression profiling corroborated by functional experiments showed that HL induced photoinhibition in CAT1AS plants and that a short-term HL exposure of CAT1AS plants triggered an increased tolerance against a subsequent severe oxidative stress.Entities:
Mesh：
Substances：
Year: 2003 PMID： 14671332 PMCID： PMC307701 DOI： 10.1073/pnas.2136610100
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205