Literature DB >> 1466909

A comparison of the different methods available for determining BCG-macrophage interactions in vitro, including a new method of colony counting in broth.

N Fazal1, R Bartlett, D A Lammas, D S Kumararatne.   

Abstract

Different methods of determining BCG viability based on colony forming unit (CFU) counting and radio-isotope labelling were comparatively assessed. These included radio-isotope labelling with [3H]uracil, [3H]uridine, [3H]glycerol, and CFU counting, by both agar plate dilution, and microcolony counting in broth. The sensitivity ranges of the different techniques were determined in both macrophage-free and macrophage-treated systems and used to assess the anti-mycobacterial potential of human monocyte-derived macrophages following BCG infection.

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Year:  1992        PMID： 1466909     DOI： 10.1111/j.1574-6968.1992.tb05921.x

Source DB:  PubMed          Journal:  FEMS Microbiol Immunol        ISSN： 0920-8534


  7 in total

1.  Microarray analysis of Mycobacterium tuberculosis-infected monocytes reveals IL26 as a new candidate gene for tuberculosis susceptibility.

Authors:  José M Guerra-Laso; Sara Raposo-García; Silvia García-García; Cristina Diez-Tascón; Octavio M Rivero-Lezcano
Journal:  Immunology       Date:  2015-02       Impact factor: 7.397

2.  CCL20 is overexpressed in Mycobacterium tuberculosis-infected monocytes and inhibits the production of reactive oxygen species (ROS).

Authors:  O M Rivero-Lezcano; C González-Cortés; D Reyes-Ruvalcaba; C Diez-Tascón
Journal:  Clin Exp Immunol       Date:  2010-09-01       Impact factor: 4.330

3.  Effects of mycobacteria on regulation of apoptosis in mononuclear phagocytes.

Authors:  K Klingler; K M Tchou-Wong; O Brändli; C Aston; R Kim; C Chi; W N Rom
Journal:  Infect Immun       Date:  1997-12       Impact factor: 3.441

4.  Rapid, efficient detection and drug susceptibility testing of Mycobacterium tuberculosis in sputum by microscopic observation of broth cultures. The Tuberculosis Working Group in Peru.

Authors:  L Caviedes; T S Lee; R H Gilman; P Sheen; E Spellman; E H Lee; D E Berg; S Montenegro-James
Journal:  J Clin Microbiol       Date:  2000-03       Impact factor: 5.948

5.  Lysis of human macrophages by cytolytic CD4+ T cells fails to affect survival of intracellular Mycobacterium bovis-bacille Calmette-Guérin (BCG).

Authors:  N Fazal; D A Lammas; M Rahelu; A D Pithie; J S Gaston; D S Kumararatne
Journal:  Clin Exp Immunol       Date:  1995-01       Impact factor: 4.330

6.  Differential effects of control and antigen-specific T cells on intracellular mycobacterial growth.

Authors:  S Worku; D F Hoft
Journal:  Infect Immun       Date:  2003-04       Impact factor: 3.441

7.  Macrophages from elders are more permissive to intracellular multiplication of Mycobacterium tuberculosis.

Authors:  José M Guerra-Laso; Sandra González-García; Carolina González-Cortés; Cristina Diez-Tascón; Ramiro López-Medrano; Octavio M Rivero-Lezcano
Journal:  Age (Dordr)       Date:  2012-07-12
  7 in total

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