PURPOSE: The objective was to describe the results of the injection of immotile spermatozoa with flexible tails when only immotile spermatozoa are present in the semen sample. METHODS: A retrospective study was conducted to analyze the procedure results for 10 couples who participated in our intracytoplasmic sperm injection program. The sperm tail was considered flexible when it moved up and down independently of the head movement, and it was considered inflexible when the movement occurred together (tail plus head). The fertilization and pregnancy rate were analyzed. RESULTS: The normal fertilization rate (presence of 2 pronuclei) was 30.3% (40/132), and the abnormal fertilization rate (presence of less than or more than 2 pronuclei) was 6.81% (9/132). A total of 52 embryos were obtained with 9 transfer procedures performed (pregnancy rate: 11.12%). CONCLUSIONS: The sperm tail flexibility test (STFT) is an easy and cost-effective way for selecting viable immotile spermatozoa and can be used as an alternative method for determining the viability of spermatozoa. This test seems to be a simple and risk-free method when compared to the swelling test.
PURPOSE: The objective was to describe the results of the injection of immotile spermatozoa with flexible tails when only immotile spermatozoa are present in the semen sample. METHODS: A retrospective study was conducted to analyze the procedure results for 10 couples who participated in our intracytoplasmic sperm injection program. The sperm tail was considered flexible when it moved up and down independently of the head movement, and it was considered inflexible when the movement occurred together (tail plus head). The fertilization and pregnancy rate were analyzed. RESULTS: The normal fertilization rate (presence of 2 pronuclei) was 30.3% (40/132), and the abnormal fertilization rate (presence of less than or more than 2 pronuclei) was 6.81% (9/132). A total of 52 embryos were obtained with 9 transfer procedures performed (pregnancy rate: 11.12%). CONCLUSIONS: The sperm tail flexibility test (STFT) is an easy and cost-effective way for selecting viable immotile spermatozoa and can be used as an alternative method for determining the viability of spermatozoa. This test seems to be a simple and risk-free method when compared to the swelling test.
Authors: Rafael Favero Ambar; Marcello M Gava; Milton Ghirelli-Filho; Ivan H Yoshida; Thais Serzedello De Paula; Sidney Glina Journal: Arab J Urol Date: 2021-07-22
Authors: Ashok Agarwal; Rakesh K Sharma; Sajal Gupta; Florence Boitrelle; Renata Finelli; Neel Parekh; Damayanthi Durairajanayagam; Ramadan Saleh; Mohamed Arafa; Chak Lam Cho; Ala'a Farkouh; Amarnath Rambhatla; Ralf Henkel; Paraskevi Vogiatzi; Nicholas Tadros; Parviz Kavoussi; Edmund Ko; Kristian Leisegang; Hussein Kandil; Ayad Palani; Gianmaria Salvio; Taymour Mostafa; Osvaldo Rajmil; Saleem Ali Banihani; Samantha Schon; Tan V Le; Ponco Birowo; Gökhan Çeker; Juan Alvarez; Juan Manuel Corral Molina; Christopher C K Ho; Aldo E Calogero; Kareim Khalafalla; Mesut Berkan Duran; Shinnosuke Kuroda; Giovanni M Colpi; Armand Zini; Christina Anagnostopoulou; Edoardo Pescatori; Eric Chung; Ettore Caroppo; Fotios Dimitriadis; Germar-Michael Pinggera; Gian Maria Busetto; Giancarlo Balercia; Haitham Elbardisi; Hisanori Taniguchi; Hyun Jun Park; Israel Maldonado Rosas; Jean de la Rosette; Jonathan Ramsay; Kasonde Bowa; Mara Simopoulou; Marcelo Gabriel Rodriguez; Marjan Sabbaghian; Marlon Martinez; Mohamed Ali Sadighi Gilani; Mohamed S Al-Marhoon; Raghavender Kosgi; Rossella Cannarella; Sava Micic; Shinichiro Fukuhara; Sijo Parekattil; Sunil Jindal; Taha Abo-Almagd Abdel-Meguid; Yoshiharu Morimoto; Rupin Shah Journal: World J Mens Health Date: 2021-10-13 Impact factor: 5.400