Literature DB >> 14662977

Evaluation of enzyme-linked immunosorbent assays and a PCR test for detection of shiga toxins for shiga toxin-producing Escherichia coli in cattle herds.

Monserrat Segura-Alvarez1, Heinz Richter, Franz J Conraths, Lutz Geue.   

Abstract

Antigen capture enzyme-linked immunosorbent assays (ELISAs) for the detection of Stx1 and/or Stx2 in cattle feces were validated in comparison to the Vero cell cytotoxicity neutralization test (as a "gold standard") applied in the course of a monitoring program for Shiga toxin-producing Escherichia coli in German cattle herds as a prescreening test and compared to MK1/MK2 PCR as an alternative prescreening test.

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Year:  2003        PMID： 14662977      PMCID： PMC308972          DOI： 10.1128/JCM.41.12.5760-5763.2003

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  17 in total

1.  A long-term study on the prevalence of shiga toxin-producing Escherichia coli (STEC) on four German cattle farms.

Authors:  L Geue; M Segura-Alvarez; F J Conraths; T Kuczius; J Bockemühl; H Karch; P Gallien
Journal:  Epidemiol Infect       Date:  2002-08       Impact factor: 2.451

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3.  Enzyme-linked immunosorbent assay for Shiga toxin and Shiga-like toxin II using P1 glycoprotein from hydatid cysts.

Authors:  D W Acheson; G T Keusch; M Lightowlers; A Donohue-Rolfe
Journal:  J Infect Dis       Date:  1990-01       Impact factor: 5.226

4.  [Verotoxin-producing E. coli (VTEC) in feces from cattle slaughtered in Germany].

Authors:  H Richter; H Klie; M Timm; P Gallien; H Steinrück; K W Perlberg; D Protz
Journal:  Berl Munch Tierarztl Wochenschr       Date:  1997-04       Impact factor: 0.328

5.  Detection of cytomegalovirus in urine from newborns by using polymerase chain reaction DNA amplification.

Authors:  G J Demmler; G J Buffone; C M Schimbor; R A May
Journal:  J Infect Dis       Date:  1988-12       Impact factor: 5.226

6.  Rapid method to detect shiga toxin and shiga-like toxin I based on binding to globotriosyl ceramide (Gb3), their natural receptor.

Authors:  S Ashkenazi; T G Cleary
Journal:  J Clin Microbiol       Date:  1989-06       Impact factor: 5.948

7.  Vero response to a cytotoxin of Escherichia coli.

Authors:  J Konowalchuk; J I Speirs; S Stavric
Journal:  Infect Immun       Date:  1977-12       Impact factor: 3.441

8.  Inhibition of human immunodeficiency virus gene amplification by heparin.

Authors:  M Holodniy; S Kim; D Katzenstein; M Konrad; E Groves; T C Merigan
Journal:  J Clin Microbiol       Date:  1991-04       Impact factor: 5.948

9.  Quantitative microtiter cytotoxicity assay for Shigella toxin.

Authors:  M K Gentry; J M Dalrymple
Journal:  J Clin Microbiol       Date:  1980-09       Impact factor: 5.948

10.  Single primer pair for amplifying segments of distinct Shiga-like-toxin genes by polymerase chain reaction.

Authors:  H Karch; T Meyer
Journal:  J Clin Microbiol       Date:  1989-12       Impact factor: 5.948
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  6 in total

1.  Shiga Toxin-Producing E. coli in Animals: Detection, Characterization, and Virulence Assessment.

Authors:  Stefanie A Barth; Rolf Bauerfeind; Christian Berens; Christian Menge
Journal:  Methods Mol Biol       Date:  2021

2.  Rapid microarray-based genotyping of enterohemorrhagic Escherichia coli serotype O156:H25/H-/Hnt isolates from cattle and clonal relationship analysis.

Authors:  Lutz Geue; Susann Schares; Birgit Mintel; Franz J Conraths; Elke Müller; Ralf Ehricht
Journal:  Appl Environ Microbiol       Date:  2010-06-25       Impact factor: 4.792

3.  Transcriptional analysis of genes encoding Shiga toxin 2 and its variants in Escherichia coli.

Authors:  Wenlan Zhang; Martina Bielaszewska; Alexander W Friedrich; Thorsten Kuczius; Helge Karch
Journal:  Appl Environ Microbiol       Date:  2005-01       Impact factor: 4.792

4.  Prevalence and characterization of shiga toxin-producing Escherichia coli in swine feces recovered in the National Animal Health Monitoring System's Swine 2000 study.

Authors:  Pina M Fratamico; Lori K Bagi; Eric J Bush; Barbara T Solow
Journal:  Appl Environ Microbiol       Date:  2004-12       Impact factor: 4.792

5.  Analysis of the clonal relationship of shiga toxin-producing Escherichia coli serogroup O165:H25 isolated from cattle.

Authors:  Lutz Geue; Thomas Selhorst; Christina Schnick; Birgit Mintel; Franz J Conraths
Journal:  Appl Environ Microbiol       Date:  2006-03       Impact factor: 4.792

6.  Analysis of the clonal relationship of serotype O26:H11 enterohemorrhagic Escherichia coli isolates from cattle.

Authors:  Lutz Geue; Sabrina Klare; Christina Schnick; Birgit Mintel; Katharina Meyer; Franz J Conraths
Journal:  Appl Environ Microbiol       Date:  2009-09-04       Impact factor: 4.792
  6 in total

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