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Literature DB >> 14659019

Comparing cDNA and oligonucleotide array data: concordance of gene expression across platforms for the NCI-60 cancer cells.

Jae K Lee¹, Kimberly J Bussey, Fuad G Gwadry, William Reinhold, Gregory Riddick, Sandra L Pelletier, Satoshi Nishizuka, Gergely Szakacs, Jean-Phillipe Annereau, Uma Shankavaram, Samir Lababidi, Lawrence H Smith, Michael M Gottesman, John N Weinstein.

Abstract

Microarray gene-expression profiles are generally validated one gene at a time by real-time RT-PCR. We describe here a different approach based on simultaneous mutual validation of large numbers of genes using two different expression-profiling platforms. The result described here for the NCI-60 cancer cell lines is a consensus set of genes that give similar profiles on spotted cDNA arrays and Affymetrix oligonucleotide chips. Global concordance is parameterized by a 'correlation of correlations' coefficient.

Entities: CellLine Chemical Disease Gene Species

Mesh：

Year: 2003 PMID： 14659019 PMCID： PMC329421 DOI： 10.1186/gb-2003-4-12-r82

Source DB: PubMed Journal: Genome Biol ISSN： 1474-7596 Impact factor: 13.583

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