BACKGROUND: Hematology and serum biochemistry values can be useful for evaluating the health of populations of captive and free-living European brown hares (Lepus europaeus). However, current information is fragmented and incomplete. OBJECTIVE: The objective of this study was to determine normal hematology and serum chemistry values for a population of captive European brown hares and to investigate differences related to sex and age. METHODS: Routine hematology and serum chemistry values were determined for 48 captive European brown hares in Catalonia (northeastern Spain): 21 young (6 males and 15 females) and 27 adult (14 males and 13 females) hares. Blood was obtained by auricular venipuncture while animals were physically restrained. Results were expressed as the mean +/- SD, and reference intervals were calculated as the mean +/- 2SD. RESULTS: RBC count, PCV, concentrations of hemoglobin, total protein, albumin, gamma globulin, urea nitrogen, and creatinine, and alanine aminotransferase activity were significantly higher in adults than in young hares, and alkaline phosphatase activity and total bilirubin concentration were lower. Sex differences were not observed. CONCLUSIONS: Hematology and serum chemistry results obtained in this study are considered representative for healthy captive European brown hares. A single reference interval can be used for values from male and female hares, however, age-specific intervals are needed for some analytes.
BACKGROUND: Hematology and serum biochemistry values can be useful for evaluating the health of populations of captive and free-living European brown hares (Lepus europaeus). However, current information is fragmented and incomplete. OBJECTIVE: The objective of this study was to determine normal hematology and serum chemistry values for a population of captive European brown hares and to investigate differences related to sex and age. METHODS: Routine hematology and serum chemistry values were determined for 48 captive European brown hares in Catalonia (northeastern Spain): 21 young (6 males and 15 females) and 27 adult (14 males and 13 females) hares. Blood was obtained by auricular venipuncture while animals were physically restrained. Results were expressed as the mean +/- SD, and reference intervals were calculated as the mean +/- 2SD. RESULTS: RBC count, PCV, concentrations of hemoglobin, total protein, albumin, gamma globulin, ureanitrogen, and creatinine, and alanine aminotransferase activity were significantly higher in adults than in young hares, and alkaline phosphatase activity and total bilirubin concentration were lower. Sex differences were not observed. CONCLUSIONS: Hematology and serum chemistry results obtained in this study are considered representative for healthy captive European brown hares. A single reference interval can be used for values from male and female hares, however, age-specific intervals are needed for some analytes.