Literature DB >> 14655048

Recombinant expression of the larval excretory-secretory antigen TES-120 of Toxocara canis in the methylotrophic yeast Pichia pastoris.

Mun-Yik Fong1, Yee-Ling Lau.   

Abstract

A gene encoding the larval excretory-secretory antigen TES-120 of the dog ascarid worm Toxocara canis was cloned into the methylotrophic yeast Pichia pastoris. Specificity of the recombinant TES-120 antigen produced by the yeast was investigated. Forty-five human serum samples from patients infected with different()parasitic organisms, including 8 cases of toxocariasis, were tested against the recombinant antigen in immunoblot assays. Results from the assays showed that the recombinant TES-120 antigen reacted with sera from toxocariasis patients only. This highly specific recombinant TES-120 antigen can potentially be used for the development of an inexpensive serodiagnostic assay for human toxocariasis.

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Year:  2003        PMID： 14655048     DOI： 10.1007/s00436-003-1020-5

Source DB:  PubMed          Journal:  Parasitol Res        ISSN： 0932-0113            Impact factor:   2.289


  13 in total

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Journal:  J Clin Microbiol       Date:  2000-04       Impact factor: 5.948

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Journal:  Parasite Immunol       Date:  1984-01       Impact factor: 2.280

7.  An abundantly expressed mucin-like protein from Toxocara canis infective larvae: the precursor of the larval surface coat glycoproteins.

Authors:  D Gems; R M Maizels
Journal:  Proc Natl Acad Sci U S A       Date:  1996-02-20       Impact factor: 11.205

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Journal:  J Clin Microbiol       Date:  1991-09       Impact factor: 5.948

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  8 in total

1.  Development and evaluation of a sensitive and specific assay for diagnosis of human toxocariasis by use of three recombinant antigens (TES-26, TES-30USM, and TES-120).

Authors:  Suharni Mohamad; Norhaida Che Azmi; Rahmah Noordin
Journal:  J Clin Microbiol       Date:  2009-04-15       Impact factor: 5.948

2.  An alternative method for producing Toxocara canis second stage larvae from a paratenic host (pigeon) for mRNA extraction purpose.

Authors:  Amir Rahbar; Alireza Alborzi; Masoudreza Seifi Abad Shapoori
Journal:  J Parasit Dis       Date:  2013-06-07

3.  Production of Toxocara cati TES-120 Recombinant Antigen and Comparison with its T. canis Homolog for Serodiagnosis of Toxocariasis.

Authors:  Farzaneh Zahabiun; Seyed Mahmoud Sadjjadi; Muhammad Hafiznur Yunus; Anizah Rahumatullah; Mohammad Hosein Falaki Moghaddam; Syazwan Saidin; Rahmah Noordin
Journal:  Am J Trop Med Hyg       Date:  2015-06-01       Impact factor: 2.345

4.  Prevalence of Anti-Toxocara canis Antibodies in Dogs Detected with Recombinant Cathepsin L-1 and TES-26 Antigens in Three States of India.

Authors:  Ashwathappa Nandini; Anju Varghese; Christophe Angeline Felicia Bora; Chundayil Kalarickal Deepa; Lanchalung Malangmei; Opinder Krishen Raina; Med Ram Verma; Karapparambu Gopalan Ajith Kumar; Lijo John; Muhasin Asaf; Gatchanda Shravan Kumar; Prabodh Kumar Hembram; Reghu Ravindran
Journal:  Acta Parasitol       Date:  2021-08-28       Impact factor: 1.440

5.  Toxocara canis mucins among other excretory-secretory antigens induce in vitro secretion of cytokines by mouse splenocytes.

Authors:  Ewa Długosz; Katarzyna Wasyl; Maciej Klockiewicz; Marcin Wiśniewski
Journal:  Parasitol Res       Date:  2015-06-06       Impact factor: 2.289

6.  Cytokine production and signalling in human THP-1 macrophages is dependent on Toxocara canis glycans.

Authors:  Ewa Długosz; Katarzyna Basałaj; Anna Zawistowska-Deniziak
Journal:  Parasitol Res       Date:  2019-08-08       Impact factor: 2.289

7.  The non-glycosylated protein of Toxocara canis MUC-1 interacts with proteins of murine macrophages.

Authors:  Rongqiong Zhou; Hongguo Jia; Zhendong Du; Aiyun Jiang; Zhenhui Song; Tao Wang; Aifang Du; Robin B Gasser; Guangxu Ma
Journal:  PLoS Negl Trop Dis       Date:  2022-09-02

8.  A new ELISA and western blot technique based on recombinant TES antigen and/or larval antigen for the detection of toxocariasis in humans.

Authors:  Marie-Kristin Raulf; Daniela Jordan; Herbert Auer; Jens M Warnecke; Bernd Lepenies; Christina Strube
Journal:  Parasitology       Date:  2020-10-23       Impact factor: 3.234
  8 in total

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