Literature DB >> 14652017

Sialyl-Tn antigen expression and O-linked GalNAc-Thr synthesis by Trypanosoma cruzi.

Teresa Freire1, Carlos Robello, Silvia Soulé, Fernando Ferreira, Eduardo Osinaga.   

Abstract

Most Trypanosoma cruzi O-glycans are linked to Thr/Ser residues via N-acetylglucosamine. We report that the mucin-type carcinoma-associated sialyl-Tn antigen (NeuAc-GalNAc-O-Ser/Thr) is expressed by T. cruzi. A specific MAb allowed us to localize the antigen on the surface of epimastigotes and to identify reactive components in parasite lysates (32, 60, and 94kDa). In addition, ppGalNAc-T activity was characterized in epimastigotes, and direct evidence was obtained for the in vitro incorporation of GalNAc to a synthetic peptide derived from a T. cruzi mucin. These results add an as yet unknown complexity to the pathways of O-glycan biosynthesis in this protozoan parasite.

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Year:  2003        PMID： 14652017     DOI： 10.1016/j.bbrc.2003.11.060

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  7 in total

1.  Module based antibody engineering: a novel synthetic REDantibody.

Authors:  Anatoliy Markiv; Bernard Anani; Ravi V Durvasula; Angray S Kang
Journal:  J Immunol Methods       Date:  2010-11-03       Impact factor: 2.303

2.  Conservation of peptide acceptor preferences between Drosophila and mammalian polypeptide-GalNAc transferase ortholog pairs.

Authors:  Thomas A Gerken; Kelly G Ten Hagen; Oliver Jamison
Journal:  Glycobiology       Date:  2008-07-31       Impact factor: 4.313

3.  Molecular analysis of a UDP-GlcNAc:polypeptide alpha-N-acetylglucosaminyltransferase implicated in the initiation of mucin-type O-glycosylation in Trypanosoma cruzi.

Authors:  Norton Heise; Divyendu Singh; Hanke van der Wel; Slim O Sassi; Jennifer M Johnson; Christa L Feasley; Carolina M Koeller; Jose O Previato; Lucia Mendonça-Previato; Christopher M West
Journal:  Glycobiology       Date:  2009-05-25       Impact factor: 4.313

4.  Glycopeptide-preferring polypeptide GalNAc transferase 10 (ppGalNAc T10), involved in mucin-type O-glycosylation, has a unique GalNAc-O-Ser/Thr-binding site in its catalytic domain not found in ppGalNAc T1 or T2.

Authors:  Cynthia L Perrine; Anjali Ganguli; Peng Wu; Carolyn R Bertozzi; Timothy A Fritz; Jayalakshmi Raman; Lawrence A Tabak; Thomas A Gerken
Journal:  J Biol Chem       Date:  2009-05-21       Impact factor: 5.157

5.  UDP-N-acetyl-α-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase from the snail Biomphalaria glabrata - structural reflections.

Authors:  Aysegül Turupcu; Peter Poliak; Christian Margreitter; Chris Oostenbrink; Erika Staudacher
Journal:  Glycoconj J       Date:  2019-08-08       Impact factor: 3.009

Review 6.  Targeting Tumor Glycans for Cancer Therapy: Successes, Limitations, and Perspectives.

Authors:  Nora Berois; Alvaro Pittini; Eduardo Osinaga
Journal:  Cancers (Basel)       Date:  2022-01-27       Impact factor: 6.639

7.  Expression and characterization of the first snail-derived UDP-N-acetyl-α-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase.

Authors:  Christopher Taus; Chantal Lucini; Takeshi Sato; Kiyoshi Furukawa; Reingard Grabherr; Erika Staudacher
Journal:  Glycoconj J       Date:  2013-07-23       Impact factor: 2.916
  7 in total

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