OBJECTIVE: Reactive oxygen species, including superoxide anions, are thought to play an important role in impairing wound healing. Additionally, superoxide anions react with nitric oxide produced by macrophages to form peroxynitrite, another strong oxidant with detrimental effects on surrounding tissue. This in vitro study investigated whether samples of metal ions and citric acid are able to reduce levels of reactive oxygen species. METHOD: Samples of materials were tested in assays for the following: inhibition of reactive oxygen species production by human polymorphonuclear neutrophils (PMNs); antioxidant activity (scavenging of superoxide anions in a cell-free system); inhibition of human complement (limiting the generation of complement factors that attract and stimulate PMNs, thereby reducing levels of reactive oxygen species). RESULTS: Metal ions were shown to inhibit both PMN production of reactive oxygen species and the activation of complement via the classical pathway, whereas citric acid was found to be a scavenger of superoxide anions. CONCLUSION: The beneficial effects of using formulations containing metal ions and citric acid on chronic wounds may be explained in part by a reduction of reactive oxygen species in these wounds.
OBJECTIVE: Reactive oxygen species, including superoxide anions, are thought to play an important role in impairing wound healing. Additionally, superoxide anions react with nitric oxide produced by macrophages to form peroxynitrite, another strong oxidant with detrimental effects on surrounding tissue. This in vitro study investigated whether samples of metal ions and citric acid are able to reduce levels of reactive oxygen species. METHOD: Samples of materials were tested in assays for the following: inhibition of reactive oxygen species production by human polymorphonuclear neutrophils (PMNs); antioxidant activity (scavenging of superoxide anions in a cell-free system); inhibition of human complement (limiting the generation of complement factors that attract and stimulate PMNs, thereby reducing levels of reactive oxygen species). RESULTS: Metal ions were shown to inhibit both PMN production of reactive oxygen species and the activation of complement via the classical pathway, whereas citric acid was found to be a scavenger of superoxide anions. CONCLUSION: The beneficial effects of using formulations containing metal ions and citric acid on chronic wounds may be explained in part by a reduction of reactive oxygen species in these wounds.
Authors: Stephan A Müller; Anja van der Smissen; Margarete von Feilitzsch; Ulf Anderegg; Stefan Kalkhof; Martin von Bergen Journal: J Mater Sci Mater Med Date: 2012-09-19 Impact factor: 3.896
Authors: M van Rossum; D P P Vooijs; X F Walboomers; M J Hoekstra; P H M Spauwen; J A Jansen Journal: J Mater Sci Mater Med Date: 2007-03-27 Impact factor: 3.896
Authors: Ayrat M Ziganshin; Elvira E Ziganshina; James Byrne; Robin Gerlach; Ellen Struve; Timur Biktagirov; Alexander Rodionov; Andreas Kappler Journal: AMB Express Date: 2015-02-01 Impact factor: 3.298