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Literature DB >> 14645594

Neutralization of enteric coronaviruses with Escherichia coli cells expressing single-chain Fv-autotransporter fusions.

Esteban Veiga¹, Víctor De Lorenzo, Luis Angel Fernández.

Abstract

We report here that fusions of single-chain antibodies (scFvs) to the autotransporter beta domain of the IgA protease of Neisseria gonorrhoeae are instrumental in locating virus-neutralizing activity on the cell surface of Escherichia coli. E. coli cells displaying scFvs against the transmissible gastroenteritis coronavirus on their surface blocked in vivo the access of the infectious agent to cultured epithelial cells. This result raises prospects for antiviral strategies aimed at hindering the entry into target cells by bacteria that naturally colonize the same intestinal niches.

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Year: 2003 PMID： 14645594 PMCID： PMC296075 DOI： 10.1128/jvi.77.24.13396-13398.2003

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

22 in total

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12 in total

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Journal: Virology Date: 2014-12-05 Impact factor: 3.616

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