BACKGROUND/AIMS: CD103, a mucosal integrin alphaEbeta7, binds to E-cadherin expressed on hepatocytes and bile duct epithelium in the liver. Although CD103+ T cells are enriched in intestinal intraepithelial lymphocytes, the localization of those cells in the liver is unknown. METHODS: We investigated whether CD103+ cells are present in human livers, and how they are associated with the intrahepatic development of T cells by flow cytometry and immunohistochemistry. RESULTS: Human livers contain significantly (P<0.001) higher percentages of CD103+ cells in CD4+ and CD8+ T cells (25.7+/-13.5 and 27.1+/-19.3%, respectively) than peripheral blood lymphocytes. Moreover, CD103+ cells in the liver, but not in peripheral blood, contained T cells with intermediate expression level of T cell receptor alphabeta. Those cells consist of mostly CD4+ and CD4-CD8- cells, and expressed low level of CD56 and interleukin-2 receptor beta chain in most of the population. These characteristics are distinct from natural killer T cells, which have been thought to be extrathymic T cells in human livers. Moreover, intrahepatic CD103+ cells expressed mRNA for recombination-activating gene-1, -2 and pre T cell receptor-alpha detected by reverse transcription-polymerase chain reaction. CONCLUSIONS: CD103+ T cells are preferentially accumulated in human livers, and those T cells show characteristics of extrathymic T cells.
BACKGROUND/AIMS: CD103, a mucosal integrin alphaEbeta7, binds to E-cadherin expressed on hepatocytes and bile duct epithelium in the liver. Although CD103+ T cells are enriched in intestinal intraepithelial lymphocytes, the localization of those cells in the liver is unknown. METHODS: We investigated whether CD103+ cells are present in human livers, and how they are associated with the intrahepatic development of T cells by flow cytometry and immunohistochemistry. RESULTS:Human livers contain significantly (P<0.001) higher percentages of CD103+ cells in CD4+ and CD8+ T cells (25.7+/-13.5 and 27.1+/-19.3%, respectively) than peripheral blood lymphocytes. Moreover, CD103+ cells in the liver, but not in peripheral blood, contained T cells with intermediate expression level of T cell receptor alphabeta. Those cells consist of mostly CD4+ and CD4-CD8- cells, and expressed low level of CD56 and interleukin-2 receptor beta chain in most of the population. These characteristics are distinct from natural killer T cells, which have been thought to be extrathymic T cells in human livers. Moreover, intrahepatic CD103+ cells expressed mRNA for recombination-activating gene-1, -2 and pre T cell receptor-alpha detected by reverse transcription-polymerase chain reaction. CONCLUSIONS:CD103+ T cells are preferentially accumulated in human livers, and those T cells show characteristics of extrathymic T cells.
Authors: Kathleen A Kelly; Dorothy Wiley; Ed Wiesmeier; Michael Briskin; Anthony Butch; Toni Darville Journal: Am J Reprod Immunol Date: 2009-04-22 Impact factor: 3.886
Authors: Femke Stelma; Annikki de Niet; Marjan J Sinnige; Karel A van Dort; Klaas P J M van Gisbergen; Joanne Verheij; Ester M M van Leeuwen; Neeltje A Kootstra; Hendrik W Reesink Journal: Sci Rep Date: 2017-07-21 Impact factor: 4.379
Authors: Marie Mura; Pinyi Lu; Tanmaya Atre; Jessica S Bolton; Elizabeth H Duncan; Sidhartha Chaudhury; Elke S Bergmann-Leitner Journal: Vaccines (Basel) Date: 2022-01-14