PURPOSE: To investigate the expression of specific receptors, signal transducers and the effect of transforming growth factor-beta (TGF-beta) on retinal pigment epithelium (RPE) migration and proliferation. METHODS: Human RPE cell line D407 was used in all experiments. The effect of TGF-beta on migration and proliferation were studied using a wound healing model and [3H]-thymidine incorporation, respectively. The expression of RNA related to the TGF-beta superfamily receptors and SMAD1-4 were assayed by reverse transcriptase-polymerase chain reaction (RT-CPR). The effects of TGF-beta on the intracellular position of SMAD were studied by immunoperoxidase and immunofluorescence. RESULTS: Transforming growth factor-beta 4 nm and activin A 0.36 nm stimulated RPE migration. There was no effect on proliferation. RNA for TGF-beta receptors types 1 and 2, and SMAD1-4 were detected in RPE culture. Transforming growth factor-beta signal transducer SMAD2 but not SMAD1 moved from the cytoplasm to the nucleus after TGF-beta stimulation. CONCLUSION: Transforming growth factor-beta can regulate RPE cell migration through specific signal transduction pathways.
PURPOSE: To investigate the expression of specific receptors, signal transducers and the effect of transforming growth factor-beta (TGF-beta) on retinal pigment epithelium (RPE) migration and proliferation. METHODS:Human RPE cell line D407 was used in all experiments. The effect of TGF-beta on migration and proliferation were studied using a wound healing model and [3H]-thymidine incorporation, respectively. The expression of RNA related to the TGF-beta superfamily receptors and SMAD1-4 were assayed by reverse transcriptase-polymerase chain reaction (RT-CPR). The effects of TGF-beta on the intracellular position of SMAD were studied by immunoperoxidase and immunofluorescence. RESULTS:Transforming growth factor-beta 4 nm and activin A 0.36 nm stimulated RPE migration. There was no effect on proliferation. RNA for TGF-beta receptors types 1 and 2, and SMAD1-4 were detected in RPE culture. Transforming growth factor-beta signal transducer SMAD2 but not SMAD1 moved from the cytoplasm to the nucleus after TGF-beta stimulation. CONCLUSION:Transforming growth factor-beta can regulate RPE cell migration through specific signal transduction pathways.
Authors: Joseph Ho; Andre J Witkin; Jonathan Liu; Yueli Chen; James G Fujimoto; Joel S Schuman; Jay S Duker Journal: Ophthalmology Date: 2010-11-20 Impact factor: 12.079
Authors: Annal D Meleth; Brian C Toy; Divya Nigam; Elvira Agrón; Robert P Murphy; Emily Y Chew; Wai T Wong Journal: Retina Date: 2013-04 Impact factor: 4.256
Authors: Joseph G Christenbury; Francisco A Folgar; Rachelle V O'Connell; Stephanie J Chiu; Sina Farsiu; Cynthia A Toth Journal: Ophthalmology Date: 2013-01-23 Impact factor: 12.079