Literature DB >> 14640719

Magnetoelastic immunosensors: amplified mass immunosorbent assay for detection of Escherichia coli O157:H7.

Chuanmin Ruan1, Kefeng Zeng, Oomman K Varghese, Craig A Grimes.   

Abstract

A mass-sensitive magnetoelastic immunosensor for detection of Escherichia coli O157:H7 is described, based on immobilization of affinity-purified antibodies attached to the surface of a micrometer-scale magnetoelastic cantilever. Alkaline phosphatase is used as a labeled enzyme to the anti-E. coli O157:H7 antibody, amplifying the mass change associated with the antibody-antigen binding reaction by biocatalytic precipitation of 5-bromo-4-chloro-3-indolyl phosphate in a pH 10.0 PBS solution. The detection limit of the biosensor is 10(2) E. coli O157:H7 cells/mL. A linear change in the resonance frequency of the biosensor was found to E. coli O157:H7 concentrations ranging from 10(2) to 10(6) cells/mL.

Entities:  

Mesh：

Year:  2003        PMID： 14640719     DOI： 10.1021/ac034562n

Source DB:  PubMed          Journal:  Anal Chem        ISSN： 0003-2700            Impact factor:   6.986


  8 in total

Review 1.  Current and developing technologies for monitoring agents of bioterrorism and biowarfare.

Authors:  Daniel V Lim; Joyce M Simpson; Elizabeth A Kearns; Marianne F Kramer
Journal:  Clin Microbiol Rev       Date:  2005-10       Impact factor: 26.132

2.  Magnetoelastic vibrational biomaterials for real-time monitoring and modulation of the host response.

Authors:  E Vlaisavljevich; H R Holmes; E L Tan; Z Qian; S Trierweiler; K G Ong; R M Rajachar
Journal:  J Mater Sci Mater Med       Date:  2013-01-26       Impact factor: 3.896

3.  A Wireless Embedded Sensor based on Magnetic Higher-order Harmonic Fields: Application to Liquid Pressure Monitoring.

Authors:  Ee Lim Tan; Brandon D Pereles; Keat Ghee Ong
Journal:  IEEE Sens J       Date:  2010-06-01       Impact factor: 3.301

4.  Wireless, remote-query, and high sensitivity Escherichia coli O157:H7 biosensor based on the recognition action of concanavalin A.

Authors:  Qingzhu Lu; Hailan Lin; Shutian Ge; Shenglian Luo; Qingyun Cai; Craig A Grimes
Journal:  Anal Chem       Date:  2009-07-15       Impact factor: 6.986

Review 5.  Biosensing technologies for Mycobacterium tuberculosis detection: status and new developments.

Authors:  Lixia Zhou; Xiaoxiao He; Dinggeng He; Kemin Wang; Dilan Qin
Journal:  Clin Dev Immunol       Date:  2011-03-16

Review 6.  Theory, instrumentation and applications of magnetoelastic resonance sensors: a review.

Authors:  Craig A Grimes; Somnath C Roy; Sanju Rani; Qingyun Cai
Journal:  Sensors (Basel)       Date:  2011-03-02       Impact factor: 3.576

7.  Antibody-based sensors: principles, problems and potential for detection of pathogens and associated toxins.

Authors:  Barry Byrne; Edwina Stack; Niamh Gilmartin; Richard O'Kennedy
Journal:  Sensors (Basel)       Date:  2009-06-05       Impact factor: 3.576

8.  Accurate Determination of the Q Quality Factor in Magnetoelastic Resonant Platforms for Advanced Biological Detection.

Authors:  Ana Catarina Lopes; Ariane Sagasti; Andoni Lasheras; Virginia Muto; Jon Gutiérrez; Dimitris Kouzoudis; José Manuel Barandiarán
Journal:  Sensors (Basel)       Date:  2018-03-16       Impact factor: 3.576
  8 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.