Shuanzeng Wei1, Tonghua Liu, Hongrui Liu, Jie Gao. 1. Department of Pathology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Science, Beijing, China.
Abstract
OBJECTIVE: To investigate the K-ras mutation pattern in six pancreatic cancer cell lines from Chinese patients. METHODS: All six cell lines were analyzed for mutation in exon 1 of the K-ras gene by polymerase chain reaction (PCR) and direct sequencing. RESULTS: All 6 pancreatic cancer cell lines had GGT --> GTT mutation at K-ras codon 12 but no mutations at codon 13. CONCLUSION: The unique GGT --> GTT mutation at codon 12 plays a potential role in the carcinogenesis of pancreatic cancers in Chinese.
OBJECTIVE: To investigate the K-ras mutation pattern in six pancreatic cancer cell lines from Chinese patients. METHODS: All six cell lines were analyzed for mutation in exon 1 of the K-ras gene by polymerase chain reaction (PCR) and direct sequencing. RESULTS: All 6 pancreatic cancer cell lines had GGT --> GTT mutation at K-ras codon 12 but no mutations at codon 13. CONCLUSION: The unique GGT --> GTT mutation at codon 12 plays a potential role in the carcinogenesis of pancreatic cancers in Chinese.