| Literature DB >> 14639654 |
Omar Kabbarah1, Mary Ann Mallon, John D Pfeifer, Winfried Edelmann, Raju Kucherlapati, Paul J Goodfellow.
Abstract
A substantial fraction of human malignancies lack functional DNA mismatch repair (MMR), accumulate mutations at high frequency, and exhibit microsatellite instability (MSI). In order to distinguish between MMR-competent and MMR-deficient malignancies, a consensus panel of microsatellite repeats has been adopted for MSI analysis of human tumors. There is no reference panel of repeats for MSI typing of murine malignancies. In this study, we present six new microsatellite repeat markers for MSI typing of mouse tumors. Analysis of polymerase chain reaction (PCR)-amplified tumor DNA from MMR-deficient and -proficient mice on denaturing polyacrylamide gels revealed that the new panel of microsatellites was more sensitive in detecting MSI than six commonly used CA(n) repeats. Using the new set of microsatellite markers, we demonstrated the presence of MSI in endometrial carcinoma and cancer precursors from diethylstilbestrol (DES)-treated mice, pointing to a possible role for loss of MMR in hormonally promoted endometrial tumorigenesis. Copyright 2003 Wiley-Liss, Inc.Entities:
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Year: 2003 PMID： 14639654 DOI： 10.1002/mc.10157
Source DB: PubMed Journal: Mol Carcinog ISSN： 0899-1987 Impact factor: 4.784