G Wang1, L Nie, H Tan. 1. Department of Molecular Microbiology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
Abstract
AIMS: To clone and characterize sanO, a gene involved in the biosynthesis of nikkomycins in Streptomyces ansochromogenes. METHODS AND RESULTS: A 4.5-kb BamHI-KpnI fragment was cloned and sequenced. Sequence analysis revealed that this fragment contains three complete open reading frames. The largest one with 2034 bp was designated sanO, which encodes a protein consisting of 667 amino acids with high similarity to module of peptide synthetase. sanO disruption mutants produced no nikkomycin X, but formed nikkomycin Z at almost the same level of the wild-type strain. The production of nikkomycin X can be recovered by genetic complementation of sanO disruption mutants. Primer extension also revealed that transcription start point(tsp) of sanO was localized 87 bp upstream of the potential start codon (GTG). CONCLUSIONS: sanO was essential for the biosynthesis of nikkomycin X. SIGNIFICANCE AND IMPACT OF THE STUDY: Nikkomycins have received increased interest of study because of their prospective application in agriculture and medicine. Cloning and characterization of genes involved in the nikkomycin biosynthesis will help to elucidate the whole biosynthetic pathway of nikkomycins.
AIMS: To clone and characterize sanO, a gene involved in the biosynthesis of nikkomycins in Streptomyces ansochromogenes. METHODS AND RESULTS: A 4.5-kb BamHI-KpnI fragment was cloned and sequenced. Sequence analysis revealed that this fragment contains three complete open reading frames. The largest one with 2034 bp was designated sanO, which encodes a protein consisting of 667 amino acids with high similarity to module of peptide synthetase. sanO disruption mutants produced no nikkomycin X, but formed nikkomycin Z at almost the same level of the wild-type strain. The production of nikkomycin X can be recovered by genetic complementation of sanO disruption mutants. Primer extension also revealed that transcription start point(tsp) of sanO was localized 87 bp upstream of the potential start codon (GTG). CONCLUSIONS: sanO was essential for the biosynthesis of nikkomycin X. SIGNIFICANCE AND IMPACT OF THE STUDY: Nikkomycins have received increased interest of study because of their prospective application in agriculture and medicine. Cloning and characterization of genes involved in the nikkomycin biosynthesis will help to elucidate the whole biosynthetic pathway of nikkomycins.