| Literature DB >> 14631119 |
June L Traicoff1, Laura De Marchis, Britten L Ginsburg, Rodolfo E Zamora, Nada H Khattar, Vincent J Blanch, Sarah Plummer, Sharon A Bargo, Dennis J Templeton, Graham Casey, Charlotte S Kaetzel.
Abstract
The human cell lines VACO-235 and VACO-411 constitute a novel in vitro model of colon adenoma to carcinoma progression. By differential display RT-PCR we identified a transcript that is expressed in the parental nontumorigenic adenoma line (VACO-235E), but is not expressed in the tumorigenic daughter (VACO-235L) or granddaughter (VACO-411) lines. This cDNA represents a previously uncharacterized portion of the 3'UTR of human PIGR. Human PIGR mRNA was found to be highly expressed in normal colon epithelium, but was decreased in 6 of 8 colon tumors and was negligible in 8 of 10 colon tumor cell lines. We sequenced the entire 1.8 kb 3'UTR of human PIGR, and found it to contain multiple repetitive elements as well as elements that could affect the processing and stability of PIGR mRNA. We hypothesize that differential regulation of PIGR mRNA stability may contribute to its downregulation in colon cancer. Copyright 2003 National Science Council, ROC and S. Karger AG, BaselEntities:
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Year: 2003 PMID： 14631119 DOI： 10.1159/000073967
Source DB: PubMed Journal: J Biomed Sci ISSN： 1021-7770 Impact factor: 8.410