| Literature DB >> 14630348 |
Yoshihito Fujita1, Naoki Yamamoto, Kazuya Sobue, Masaaki Inagaki, Hiroaki Ito, Hajime Arima, Tetsuro Morishima, Akinori Takeuchi, Takako Tsuda, Hirotada Katsuya, Kiyofumi Asai.
Abstract
Aquaporins (AQPs) are a family of water-selective transporting proteins with homology to the major intrinsic protein (MIP) of lens, that increase plasma membrane water permeability in secretory and absorptive cells. In this study, we investigated the effect of mild hypothermia on the expression of AQP4, AQP5 and AQP9 in rat astrocytes cultured under hypoxic conditions. At 37 degrees C, a marked decrease in the expression of AQP4, AQP5 and AQP9 mRNAs was observed. However, at 32 degrees C (mild hypothermia), the expression of AQP5 mRNA was restored to its basal level. Interestingly, under mild hypothermia AQP4 mRNA expression transiently decreased and then increased about two-fold; while AQP9 mRNA expression decreased the same as at 37 degrees C. The changes in the expression of AQP4 and AQP9 proteins were confirmed by Western blot analysis. The restoration of the AQP4 and AQP5 expression at 32 degrees C from the hypoxia-induced decrease at 37 degrees C may play an important role in the reduction of brain edema under hypothermic conditions.Entities:
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Year: 2003 PMID： 14630348 DOI： 10.1016/j.neures.2003.08.006
Source DB: PubMed Journal: Neurosci Res ISSN： 0168-0102 Impact factor: 3.304