Literature DB >> 14630338

Collection and characterisation of bacterial membrane proteins.

Massoud Saidijam1, Georgios Psakis, Joanne L Clough, Johan Meuller, Shun'ichi Suzuki, Christopher J Hoyle, Sarah L Palmer, Scott M Morrison, Martin K Pos, Richard C Essenberg, Martin C J Maiden, Atif Abu-bakr, Simon G Baumberg, Alex A Neyfakh, Jeffrey K Griffith, Michael J Stark, Alison Ward, John O'Reilly, Nicholas G Rutherford, Mary K Phillips-Jones, Peter J F Henderson.   

Abstract

A general strategy for the amplified expression in Escherichia coli of membrane transport and receptor proteins from other bacteria is described. As an illustration we report the cloning of the putative alpha-ketoglutarate membrane transport gene from the genome of Helicobacter pylori, overexpression of the protein tagged with RGS(His)6 at the C-terminus, and its purification in mg quantities. The retention of structural and functional integrity was verified by circular dichroism spectroscopy and reconstitution of transport activity. This strategy for overexpression and purification is extended to additional membrane proteins from H. pylori and from other bacteria.

Entities:  

Mesh：

Substances：

Year:  2003        PMID： 14630338     DOI： 10.1016/s0014-5793(03)01148-7

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  9 in total

1.  The hydantoin transport protein from Microbacterium liquefaciens.

Authors:  Shun'ichi Suzuki; Peter J F Henderson
Journal:  J Bacteriol       Date:  2006-05       Impact factor: 3.490

2.  Characterisation of the DAACS Family Escherichia coli Glutamate/Aspartate-Proton Symporter GltP Using Computational, Chemical, Biochemical and Biophysical Methods.

Authors:  Moazur Rahman; Fouzia Ismat; Li Jiao; Jocelyn M Baldwin; David J Sharples; Stephen A Baldwin; Simon G Patching
Journal:  J Membr Biol       Date:  2016-12-26       Impact factor: 1.843

3.  Recombinant expression screening of P. aeruginosa bacterial inner membrane proteins.

Authors:  Vidya Madhavan; Forum Bhatt; Constance J Jeffery
Journal:  BMC Biotechnol       Date:  2010-11-29       Impact factor: 2.563

4.  Allantoin transport protein, PucI, from Bacillus subtilis: evolutionary relationships, amplified expression, activity and specificity.

Authors:  Pikyee Ma; Simon G Patching; Ekaterina Ivanova; Jocelyn M Baldwin; David Sharples; Stephen A Baldwin; Peter J F Henderson
Journal:  Microbiology (Reading)       Date:  2016-03-11       Impact factor: 2.777

5.  Functional characterization of the initiation enzyme of S-layer glycoprotein glycan biosynthesis in Geobacillus stearothermophilus NRS 2004/3a.

Authors:  Kerstin Steiner; René Novotny; Kinnari Patel; Evgenij Vinogradov; Chris Whitfield; Miguel A Valvano; Paul Messner; Christina Schäffer
Journal:  J Bacteriol       Date:  2007-01-19       Impact factor: 3.490

6.  Production of membrane proteins for characterisation of their pheromone-sensing and antimicrobial resistance functions.

Authors:  Aalishaa A Azam; Jean M Kinder; G Nasir Khan; Ade Alase; Pikyee Ma; Yang Liu; James R Ault; Peter J F Henderson; Babur Z Chowdhry; Bruce D Alexander; Stephen E Harding; Mary K Phillips-Jones
Journal:  Eur Biophys J       Date:  2018-07-31       Impact factor: 1.733

Review 7.  Purification of bacterial membrane sensor kinases and biophysical methods for determination of their ligand and inhibitor interactions.

Authors:  Rohanah Hussain; Stephen E Harding; Charlotte S Hughes; Pikyee Ma; Simon G Patching; Shalini Edara; Giuliano Siligardi; Peter J F Henderson; Mary K Phillips-Jones
Journal:  Biochem Soc Trans       Date:  2016-06-15       Impact factor: 5.407

8.  An efficient strategy for small-scale screening and production of archaeal membrane transport proteins in Escherichia coli.

Authors:  Pikyee Ma; Filipa Varela; Malgorzata Magoch; Ana Rita Silva; Ana Lúcia Rosário; José Brito; Tânia Filipa Oliveira; Przemyslaw Nogly; Miguel Pessanha; Meike Stelter; Arnulf Kletzin; Peter J F Henderson; Margarida Archer
Journal:  PLoS One       Date:  2013-10-07       Impact factor: 3.240

9.  Characterisation of the selective binding of antibiotics vancomycin and teicoplanin by the VanS receptor regulating type A vancomycin resistance in the enterococci.

Authors:  C S Hughes; E Longo; M K Phillips-Jones; R Hussain
Journal:  Biochim Biophys Acta Gen Subj       Date:  2017-05-13       Impact factor: 3.770
  9 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.