Hexaplex PCR for rapid detection of virulence factors.

PCR technology has emerged as a basic tool in biological research and in the detection of infectious organisms. It has the potential to provide information on a number of toxins and virulence factors, as well as allowing species identification of pathogens. Multiplex PCR assays are becoming prevalent for the simultaneous detection of toxins, virulence factors and pathogens in clinical and environmental specimens. This review will discuss the hexaplex PCR assay, its application and future directions.