Qun-fei Zhao1, Xue-liang Gao, Min Qian. 1. State Key Laboratory of Bio-Orgnic and Natural Product Chemistry, Shanghai Institute of Orgnic Chemistry, Chinese Academy of Science, Shanghai 200032.
Abstract
OBJECTIVE: To detect the effect of H2O2 on Acanthamoeba spp.. METHODS: By Wright's stain, quantitative culture, MTT assay and lactate dehydrogenase(LDH) assessment, the influence of H2O2 on the morphological feature, proliferation speed and the survival rate of Acanthamoeba was tested. RESULTS: At low concentration of 0.125%, H2O2 can force the Acanthamoeba trophozoites into cysts irreversibly, and inhibit its proliferation. 1% H2O2 can directly destroy Acanthamoeba trophozoites. CONCLUSION: H2O2 is effective in destroying Acanthamoeba. It is possible to be used as an ideal reagent for the prevention of Acanthamoeba keratitis.
OBJECTIVE: To detect the effect of H2O2 on Acanthamoeba spp.. METHODS: By Wright's stain, quantitative culture, MTT assay and lactate dehydrogenase(LDH) assessment, the influence of H2O2 on the morphological feature, proliferation speed and the survival rate of Acanthamoeba was tested. RESULTS: At low concentration of 0.125%, H2O2 can force the Acanthamoeba trophozoites into cysts irreversibly, and inhibit its proliferation. 1% H2O2 can directly destroy Acanthamoeba trophozoites. CONCLUSION:H2O2 is effective in destroying Acanthamoeba. It is possible to be used as an ideal reagent for the prevention of Acanthamoeba keratitis.
Authors: I Heredero-Bermejo; J L Copa-Patiño; J Soliveri; R Gómez; F J de la Mata; J Pérez-Serrano Journal: Parasitol Res Date: 2013-09-12 Impact factor: 2.289