Literature DB >> 14626416

Refolding of a recombinant full-length non-structural (NS3) protein from hepatitis C virus by chromatographic procedures.

Ming Li1, Anton Poliakov, U Helena Danielson, Zhiguo Su, Jan-Christer Janson.   

Abstract

The non-structural protein 3 (NS3) of Hepatitis C virus (HCV) was expressed as inactive aggregates in Escherichia coli. The protein was refolded by chromatographic techniques of which ion exchange chromatography was best for crude samples and gel filtration best for partially purified samples. Immobilized metal ion affinity chromatography showed intermediate performance. Gradient procedures enhanced the recovery of active NS3 protein.

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Year:  2003        PMID： 14626416     DOI： 10.1023/a:1026050627117

Source DB:  PubMed          Journal:  Biotechnol Lett        ISSN： 0141-5492            Impact factor:   2.461


  4 in total

Review 1.  Production of active eukaryotic proteins through bacterial expression systems: a review of the existing biotechnology strategies.

Authors:  Sudhir Sahdev; Sunil K Khattar; Kulvinder Singh Saini
Journal:  Mol Cell Biochem       Date:  2007-09-12       Impact factor: 3.396

2.  Isolation of cell-free bacterial inclusion bodies.

Authors:  Escarlata Rodríguez-Carmona; Olivia Cano-Garrido; Joaquin Seras-Franzoso; Antonio Villaverde; Elena García-Fruitós
Journal:  Microb Cell Fact       Date:  2010-09-17       Impact factor: 5.328

3.  An account on the innovative path of cooperation with Prof. Zhiguo Su in bioseparation.

Authors:  Jan-Christer Janson
Journal:  Eng Life Sci       Date:  2021-06-07       Impact factor: 2.678

4.  Strategies for the recovery of active proteins through refolding of bacterial inclusion body proteins.

Authors:  Luis Felipe Vallejo; Ursula Rinas
Journal:  Microb Cell Fact       Date:  2004-09-02       Impact factor: 5.328
  4 in total

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