I Takeuchi1, H Ishida, T Mori, D Hashimoto. 1. Department of Surgery, Saitama Medical Center and School, 1981 Kamoda, Kawagoe, Saitama, 350-8550, Japan. ikuya@sj8.so-net.ne.jp
Abstract
BACKGROUND: Natural killer (NK) activity plays an important role in the prevention of tumor metastasis. However, few studies compared changes in NK activities between gas(less) laparoscopy and open surgery. MATERIALS AND METHODS: Donryu rats were randomized to 4 groups ( n = 6-9, for each lime point in each group): gasless group, pneumoperitoneum group, laparotomy group, and control group. The surgical treatment groups received additional cecal resection at the end of the procedures. Spleen cells and hepatic nonparenchymal cells were harvested at 6, 24, or 96 h, to determine the splenic and hepatic NK activities against YAC-1 cells. RESULTS: Compared with the anesthesia control, laparotomy and pneumoperitoneum depressed splenic NK activity at 24 h ( p < 0.01 for laparotomy and p < 0.05, for pneumoperitoneum) and 96 h ( p < 0.05). Laparotomy also depressed hepatic NK activity at 24 h ( p < 0.05). In contrast, the gasless procedure did not show any deterioration in the splenic and hepatic NK activities. CONCLUSION: These results suggest that gasless laparoscopic surgery would be a favorable approach in terms of the preservation of NK activities.
BACKGROUND: Natural killer (NK) activity plays an important role in the prevention of tumor metastasis. However, few studies compared changes in NK activities between gas(less) laparoscopy and open surgery. MATERIALS AND METHODS: Donryu rats were randomized to 4 groups ( n = 6-9, for each lime point in each group): gasless group, pneumoperitoneum group, laparotomy group, and control group. The surgical treatment groups received additional cecal resection at the end of the procedures. Spleen cells and hepatic nonparenchymal cells were harvested at 6, 24, or 96 h, to determine the splenic and hepatic NK activities against YAC-1 cells. RESULTS: Compared with the anesthesia control, laparotomy and pneumoperitoneum depressed splenic NK activity at 24 h ( p < 0.01 for laparotomy and p < 0.05, for pneumoperitoneum) and 96 h ( p < 0.05). Laparotomy also depressed hepatic NK activity at 24 h ( p < 0.05). In contrast, the gasless procedure did not show any deterioration in the splenic and hepatic NK activities. CONCLUSION: These results suggest that gasless laparoscopic surgery would be a favorable approach in terms of the preservation of NK activities.
Authors: Marco Braga; Nicolò Pecorelli; Matteo Frasson; Andrea Vignali; Walter Zuliani; Valerio Di Carlo Journal: World J Gastrointest Oncol Date: 2011-03-15
Authors: Philipp Lingohr; Jonas Dohmen; Hanno Matthaei; Timo Schwandt; Gun-Soo Hong; Nils Konieczny; Edwin Bölke; Sven Wehner; Jörg C Kalff Journal: Eur J Med Res Date: 2014-06-17 Impact factor: 2.175