| Literature DB >> 14623081 |
Shuiyun Lan1, Hua Wang, Hong Jiang, Hongxia Mao, Xiaoying Liu, Xiaonan Zhang, Yunwen Hu, Li Xiang, Zhenghong Yuan.
Abstract
It has been suggested that cellular proteins are involved in hepatitis C virus (HCV) RNA replication. By using the yeast two-hybrid system, we isolated seven cDNA clones encoding proteins interacting with HCV RNA polymerase (NS5B) from a human liver cDNA library. For one of these, alpha-actinin, we confirmed the interaction by coimmunoprecipitation, immunofluorescent staining and confocal microscopic analysis. Experiments with deletion mutants showed that domains NS5B(84-95), NS5B(466-478), and alpha-actinin(621-733) are responsible for the interaction. Studies of the HCV subgenomic replicon system with small interference RNA indicate that alpha-actinin is essential for HCV RNA replication. Our results suggest alpha-actinin may be a component of the HCV replication complex.Entities:
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Year: 2003 PMID： 14623081 DOI： 10.1016/s0014-5793(03)01163-3
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124